Bigi A, Gandolfi M, Koch M H, Roveri N
Department of Chemistry G. Ciamician, University of Bologna, Italy.
Biomaterials. 1996 Jun;17(12):1195-201. doi: 10.1016/0142-9612(96)84940-4.
Decalcified samples of turkey leg tendon were submitted to in vitro calcification in the presence of metastable solutions of calcium phosphate at different concentrations. The structural relationship between apatitic deposits and collagen fibrils was examined by high- and small-angle X-ray diffraction using conventional and synchrotron radiation sources. At high supersaturation the apatitic crystallites were deposited on the collagen fibrils with their crystallographic c-axis preferentially oriented parallel to the fibril axis. At lower supersaturation, a fraction of the apatitic crystallites also grew with the c-axis preferentially oriented parallel to the collagen fibril axis, whereas other exhibited a preferential orientation perpendicular to the fibril axis. The analysis of the small-angle X-ray diffraction data indicates that the deposition of the apatitic phase in the sample stored in solution at lower supersaturation induced modifications of the collagen electron density distribution in the axial direction, which can be attributed to the deposition of the inorganic crystallites inside the gap region of the collagen structure.
将火鸡腿部肌腱的脱钙样本置于不同浓度的磷酸钙亚稳溶液中进行体外钙化。使用传统和同步辐射源，通过高角度和小角度X射线衍射研究了磷灰石沉积物与胶原纤维之间的结构关系。在高过饱和度下，磷灰石微晶沉积在胶原纤维上，其结晶c轴优先平行于纤维轴取向。在较低过饱和度下，一部分磷灰石微晶也以c轴优先平行于胶原纤维轴的方向生长，而其他微晶则表现出垂直于纤维轴的优先取向。小角度X射线衍射数据分析表明，在较低过饱和度下储存在溶液中的样品中磷灰石相的沉积导致了胶原电子密度分布在轴向上的改变，这可归因于无机微晶在胶原结构间隙区域内的沉积。
