杀鲑气单胞菌可转移R质粒中的磺胺抗性基因。

Sulfonamide resistance gene in a transferable R plasmid of Pasteurella piscicida.

作者信息

Kim E H, Aoki T

机构信息

Chongpyong Inland Fisheries Research Laboratory, National Fisheries Research & Development Agency, Kyeonggi-do, Korea.

出版信息

Microbiol Immunol. 1996;40(5):397-9. doi: 10.1111/j.1348-0421.1996.tb01085.x.

DOI:10.1111/j.1348-0421.1996.tb01085.x

PMID:8805105

Abstract

The sulfonamide resistance (SAr) determinant was cloned from a transferable R plasmid of Pasteurella piscicida, pSP9351, and the sequence was determined. The resistance gene (pp-sul) was localized to an approximately 1-kb region that includes the PstI-EcoRI site in the restriction map. An open reading frame coding a sul II-type gene composed of 810 nucleotides was identified. A direct repeat sequence was shown in the 5' flanking region of pp-sul, and a plasmid recombinational event may have occurred during the construction of pSP9351. In the 3' flanking region of the gene, a sequence homologous to the 5' noncoding sequence of the trimethoprim resistance gene, dhfr IX was found.

摘要

从杀鲑气单胞菌杀鲑亚种的可转移R质粒pSP9351中克隆出磺胺抗性（SAr）决定簇，并测定了其序列。抗性基因（pp-sul）定位于限制性图谱中包含PstI-EcoRI位点的约1 kb区域。鉴定出一个由810个核苷酸组成的编码sul II型基因的开放阅读框。在pp-sul的5'侧翼区域显示出一个直接重复序列，并且在pSP9351的构建过程中可能发生了质粒重组事件。在该基因的3'侧翼区域，发现了与甲氧苄啶抗性基因dhfr IX的5'非编码序列同源的序列。