马淋巴细胞对不表达主要组织相容性复合体抗原的类肉瘤衍生肿瘤细胞产生体外天然细胞毒性。

Generation of in vitro natural cytotoxicity of horse lymphocytes against sarcoid-derived tumor cells not expressing major histocompatibility complex antigens.

作者信息

Broström H, Troye-Bomberg M, Perlmann P

机构信息

Department of Medicine and Surgery, Swedish University of Agricultural Sciences, Uppsala, Sweden.

出版信息

Am J Vet Res. 1996 Jul;57(7):992-9.

PMID:8807009

Abstract

OBJECTIVE

To analyze in vitro lymphocyte-mediated immune responses of horses with sarcoids against allogeneic sarcoid cells containing endogenous retrovirus but not expressing major histocompatibility complex antigens.

DESIGN

Lymphocyte-mediated immune reactions were assessed by means of proliferative responses in mixed lymphocyte tumor cell culture (MLTC) assay and lymphocyte-mediated cytotoxicity against various equine target cells.

ANIMALS

12 horses with sarcoid tumors and 15 control horses.

PROCEDURE

Blood lymphocytes were cocultured in MLTC with allogeneic sarcoid cells (Mc-1, BayMc-1), equine testis cells, or normal equine dermal fibroblasts. Lymphocytes were assayed for proliferative responses by [3H]thymidine uptake and for cytotoxicity against the same targets by 51Cr release assay. The lymphocyte populations were analyzed for some common surface markers.

RESULTS

Lymphocytes from horses with sarcoids exerted an anamnestic proliferative response in MLTC against Mc-1 cells, but this procedure never generated cytotoxic lymphocytes. However, lymphocytes from all horses cultured in medium with 10% allogeneic serum only had selective. natural cytotoxicity against Mc-1 that was generated without DNA synthesis. Approximately 80% of the lymphocytes disappeared during culture; however the remaining population of small, viable lymphocytes indicated a decrease of CD4+ T lymphocytes, but numbers of T cells with receptors for Helix pomatia A hemagglutinin were unaffected. Few lymphocytes had Fc-receptors for IgG, were complement-reactive positive cells or were B cells expressing surface immunoglobulin.

CONCLUSIONS

Results may indicate a natural defense system, which preferentially recognizes and lyses tumor cells that are deficient in surface expression of major histocompatibility complex antigens, without intervention of conventional T-cell receptors or antibodies.

摘要

目的

分析患有肉瘤的马匹针对含有内源性逆转录病毒但不表达主要组织相容性复合体抗原的同种异体肉瘤细胞的体外淋巴细胞介导的免疫反应。

设计

通过混合淋巴细胞肿瘤细胞培养（MLTC）试验中的增殖反应以及针对各种马靶细胞的淋巴细胞介导的细胞毒性来评估淋巴细胞介导的免疫反应。

动物

12匹患有肉瘤肿瘤的马和15匹对照马。

步骤

将血液淋巴细胞与同种异体肉瘤细胞（Mc-1、BayMc-1）、马睾丸细胞或正常马皮肤成纤维细胞在MLTC中共培养。通过[³H]胸腺嘧啶核苷摄取检测淋巴细胞的增殖反应，并通过⁵¹Cr释放试验检测其对相同靶标的细胞毒性。分析淋巴细胞群体的一些常见表面标志物。

结果

患有肉瘤的马匹的淋巴细胞在MLTC中对Mc-1细胞产生了回忆性增殖反应，但该过程从未产生细胞毒性淋巴细胞。然而，仅在含有10%同种异体血清的培养基中培养的所有马匹的淋巴细胞对Mc-1具有选择性天然细胞毒性，这种毒性在没有DNA合成的情况下产生。培养过程中约80%的淋巴细胞消失；然而，剩余的小而存活的淋巴细胞群体显示CD4⁺T淋巴细胞减少，但具有对苹果螺A血凝素受体的T细胞数量未受影响。很少有淋巴细胞具有IgG的Fc受体、是补体反应阳性细胞或表达表面免疫球蛋白的B细胞。