Schnaidt M, Northoff H, Wernet D
Department of Transfusion Medicine, University Hospital Tübingen, Germany.
Transfus Med. 1996 Jun;6(2):111-4. doi: 10.1046/j.1365-3148.1996.d01-58.x.
The frequency and specificity of platelet-alloantibodies to human platelet antigens (HPA) -1, -3 and -5 was investigated in 59 multitransfused, HLA-immunized patients. Using the MAIPA test (monoclonal antibody specific immobilization of platelet antigens) platelet alloantibodies could be demonstrated in 10 (17%) patients. In one patient the antibody was present prior to any transfusions and probably induced by multiple previous pregnancies. This antibody was directed to HPA-5b. The remaining nine antibodies were found in patients (n = 36) with HLA-antibodies reacting with over 95% of unselected lymphocytes. In these patients the target antigens were HPA-1b in six, HPA-3a in one and both antigens in two patients. Our findings demonstrate platelet alloimmunization induced by transfusions to be restricted to patients with high HLA-immunization. 25% of these patients (9/36) show platelet-specific antibodies, primarily HPA-1b.
在59例多次输血且已产生HLA免疫的患者中，研究了针对人类血小板抗原（HPA）-1、-3和-5的血小板同种抗体的频率和特异性。采用MAIPA试验（血小板抗原单克隆抗体特异性固定法），在10例（17%）患者中检测到血小板同种抗体。1例患者在首次输血前就存在该抗体，可能是由于既往多次妊娠所致。该抗体针对HPA-5b。其余9例抗体见于HLA抗体与超过95%未选择淋巴细胞发生反应的患者（n = 36）。在这些患者中，6例的靶抗原为HPA-1b，1例为HPA-3a，2例为两种抗原。我们的研究结果表明，输血诱导的血小板同种免疫仅限于HLA高度免疫的患者。这些患者中有25%（9/36）出现血小板特异性抗体，主要是HPA-1b。
