Williamson K C, Fujioka H, Aikawa M, Kaslow D C
Biology Department, Loyola University, Chicago, IL 60626, USA.
Mol Biochem Parasitol. 1996 Jun;78(1-2):161-9. doi: 10.1016/s0166-6851(96)02621-7.
During Plasmodium falciparum gametocytogenesis, Pfs230, a malaria transmission-blocking vaccine candidate, is expressed as a 360 kDa protein localized to the parasitophorous vacuole/parasite plasma membrane. When gametocytes emerge from red blood cells, as they do when taken up in a blood meal by a mosquito, Pfs230 is processed from a 360 kDa form to a 310 kDa form the latter of which is exposed on the exterior surface of gametes. The 50 kDa portion of Pfs230, removed from the amino-terminus of the 360 kDa form, contains 25 contiguous glutamates and an EEVG16 repeat. Analogous to other P. falciparum proteins, the repeat region appears to be immunodominant. As the gamete emerges from the red blood cell and is exposed to the antibodies in the blood meal, cleavage of the immunodominant region of Pfs230 may contribute to an immune evasion strategy by the parasite.
在恶性疟原虫配子体发生过程中，疟疾传播阻断疫苗候选物Pfs230表达为一种360 kDa的蛋白质，定位于寄生泡/寄生虫质膜。当配子体从红细胞中释放出来时，就像被蚊子吸食血餐时那样，Pfs230从360 kDa的形式加工成310 kDa的形式，后者暴露在配子的外表面。从360 kDa形式的氨基末端去除的Pfs230的50 kDa部分包含25个连续的谷氨酸和一个EEVG16重复序列。与其他恶性疟原虫蛋白质类似，重复区域似乎具有免疫显性。当配子从红细胞中出现并暴露于血餐中的抗体时，Pfs230免疫显性区域的切割可能有助于寄生虫的免疫逃避策略。
