肝细胞生长因子对转基因小鼠肝细胞癌转移的抑制作用

Inhibitory effect of hepatocyte growth factor on metastasis of hepatocellular carcinoma in transgenic mice.

作者信息

Shiota G, Kawasaki H, Nakamura T, Schmidt E V

机构信息

Second Department of Internal Medicine, Tottori University Faculty of Medicine, Yonago, Japan.

出版信息

Res Commun Mol Pathol Pharmacol. 1996 Jan;91(1):33-9.

PMID:8824929

Abstract

Hepatocyte growth factor (HGF) has different effects on different cell types. Recently, it has been reported that HGF has an anti-proliferative effect against tumor cells including hepatocellular carcinoma (HCC) cells. To clarify whether HGF inhibits the metastasis of HCC cells in vivo, we examined the metastatic potential of HCC cells into liver where HGF was highly expressed in transgenic animals. Three HCC cell lines including HuH7, Hep3B and FaO cells were transplanted into liver through the portal vein in chimeric mice or HGF transgenic mice and athymic nude mice (HGF/nude). The incidence of liver metastasis and number of metastatic tumors were significantly lower in mice expressing HGF (HGF/nude), compared with their siblings expressing no HGF (+/nude) (p < 0.05 and p = 0.002, respectively). These data suggest that HGF really inhibits metastasis of HCC cells in vivo.

摘要

肝细胞生长因子（HGF）对不同细胞类型有不同影响。最近有报道称，HGF对包括肝细胞癌（HCC）细胞在内的肿瘤细胞具有抗增殖作用。为了阐明HGF在体内是否抑制HCC细胞的转移，我们在转基因动物肝脏中高度表达HGF的情况下，检测了HCC细胞向肝脏的转移潜能。将包括HuH7、Hep3B和FaO细胞在内的三种HCC细胞系通过门静脉移植到嵌合小鼠、HGF转基因小鼠和无胸腺裸鼠（HGF/裸鼠）的肝脏中。与不表达HGF的同窝小鼠（+/裸鼠）相比，表达HGF的小鼠（HGF/裸鼠）肝脏转移的发生率和转移瘤数量显著降低（分别为p < 0.05和p = 0.002）。这些数据表明，HGF在体内确实抑制HCC细胞的转移。