采用高效液相色谱-串联质谱法测定生物样品中的1-O-酰基-2-乙酰基-sn-甘油-3-磷酸胆碱、血小板活化因子及相关磷脂。
Determination of 1-O-acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine, platelet-activating factor and related phospholipids in biological samples by high-performance liquid chromatography--tandem mass spectrometry.
作者信息
Savu S R, Silvestro L, Sörgel F, Montrucchio G, Lupia E, Camussi G
机构信息
IBMP, Institut für Biomedizinische und Pharmazeutische Forschung, Nürnberg-Heroldsberg, Germany.
出版信息
J Chromatogr B Biomed Appl. 1996 Jun 28;682(1):35-45. doi: 10.1016/0378-4347(96)00070-9.
Combining normal-phase HPLC separation and tandem mass spectrometric detection, using an ion-spray HPLC-MS interface, a quantitative method for acyl-platelet activating factor (acyl-PAF), platelet-activating factor (PAF) and related phospholipids was developed. Mass spectra, positive ions, showed intense [M+H]+ ions; collision-induced dissociation of protonated molecular ions gave characteristic daughter ions corresponding to the polar head. Detection limits of 0.1-0.3 ng injected were obtained by multiple reaction monitoring. Samples of human endothelial cells treated with compounds modulating the levels of acyl-PAF and PAF have been analyzed by the present technique, proving that this approach is suitable for biochemical studies.
结合正相高效液相色谱分离和串联质谱检测，使用离子喷雾高效液相色谱-质谱接口，开发了一种用于酰基血小板活化因子（acyl-PAF）、血小板活化因子（PAF）及相关磷脂的定量方法。质谱图中，正离子显示出强烈的[M+H]+离子；质子化分子离子的碰撞诱导解离产生了对应于极性头部的特征性子离子。通过多反应监测获得了注入量为0.1 - 0.3 ng的检测限。用本技术分析了经调节酰基-PAF和PAF水平的化合物处理的人内皮细胞样品，证明该方法适用于生化研究。
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