Almeida-Porada G, Ascensão J L
Department of Medicine, University of Nevada School of Medicine, and the Veterans Affairs Medical Center, Reno 89520, USA.
J Lab Clin Med. 1996 Oct;128(4):399-407. doi: 10.1016/s0022-2143(96)80012-6.
Bone marrow endothelial cells (BMECs) are an integral part of the bone marrow microenvironment and are likely to play an important role in the regulation of hematopoiesis, either by producing growth factors or inhibitory cytokines or by displaying adhesion molecules that can interact with hematopoietic progenitors. In the present study we demonstrate the isolation, propagation, and characterization of BMECs with regard to morphology, growth characteristics, phenotype, and production of cytokines. Furthermore, we report the creation of a cell line with "BMEC-like" characteristics and compare the characteristics of primary BMEC cultures to those of the immortalized cell line. In addition, we demonstrate that BMECs are susceptible to infection by a laboratory strain of human cytomegalovirus (CMV), suggesting that CMV infection of endothelial cells in vivo could potentially play a role in the hematologic abnormalities observed during CMV infection.
骨髓内皮细胞（BMECs）是骨髓微环境的一个组成部分，可能通过产生生长因子或抑制性细胞因子，或通过展示可与造血祖细胞相互作用的黏附分子，在造血调节中发挥重要作用。在本研究中，我们展示了BMECs的分离、增殖以及在形态、生长特性、表型和细胞因子产生方面的特征。此外，我们报告了具有“类BMEC”特征的细胞系的建立，并将原代BMEC培养物的特征与永生化细胞系的特征进行比较。另外，我们证明BMECs易受实验室人巨细胞病毒（CMV）株的感染，这表明体内内皮细胞的CMV感染可能在CMV感染期间观察到的血液学异常中发挥作用。
