Jiang J, Tyssebotn I
Department of Physiology, University of Bergen, Norway.
Undersea Hyperb Med. 1996 Jun;23(2):99-106.
These experiments were designed to establish an animal model of acute carbon monoxide (CO) poisoning in awake habituated rats. On the day before exposure, under a brief anesthesia, a Levine preparation (unilateral common carotid artery occlusion) was performed on group 1 (n = 8) and 2 (n = 28), but not on group 3 rats (n = 8). Group 1 rats were exposed to air as control. Groups 2 and 3 rats were exposed to 0.27% CO in air for 60 min [carboxyhemoglobin (HbCO) = 70%] followed by a 2-day recovery in air. The Levine preparation per se did not induce any detectable physiologic effects on group 1 rats. Identical cardiovascular and metabolic responses to CO occurred in groups 2 and 3. After the CO exposure, all group 3 rats lived for 2 days with normal neurologic index (NI). In group 2 (n = 25 post-CO), 84% of the rats showed increased NI and edema of the ipsilateral cerebral hemisphere, and 76% of the rats died 8.7 +/- 1.7 h after the CO exposure. NI correlated with the brain edema (rs = 0.748, P < 0.001) and inversely correlated with the survival time after the CO exposure (rs = -0.777, P < 0.001). We therefore may conclude that exposure of the Levine-prepared rats to 0.27% CO in air for 60 min will provide a valuable model for testing of different treatments for CO poisoning.
这些实验旨在建立清醒习惯化大鼠急性一氧化碳（CO）中毒的动物模型。在暴露前一天，在短暂麻醉下，对第1组（n = 8）和第2组（n = 28）大鼠进行了Levine制备（单侧颈总动脉闭塞），但第3组大鼠（n = 8）未进行。第1组大鼠暴露于空气中作为对照。第2组和第3组大鼠暴露于含0.27% CO的空气中60分钟[碳氧血红蛋白（HbCO）= 70%]，随后在空气中恢复2天。Levine制备本身对第1组大鼠未诱导任何可检测到的生理效应。第2组和第3组对CO出现了相同的心血管和代谢反应。CO暴露后，所有第3组大鼠存活2天，神经指数（NI）正常。在第2组（n = 25只CO暴露后），84%的大鼠NI升高且同侧脑半球出现水肿，76%的大鼠在CO暴露后8.7±1.7小时死亡。NI与脑水肿相关（rs = 0.748，P < 0.001），与CO暴露后的存活时间呈负相关（rs = -0.777，P < 0.001）。因此，我们可以得出结论，将经过Levine制备的大鼠暴露于含0.27% CO的空气中60分钟，将为测试CO中毒的不同治疗方法提供一个有价值的模型。
