Brondz B D, Khachikian E Ia, Drizlikh G I, Andreev A V
Biull Eksp Biol Med. 1977 Jun;83(6):723-5.
Substitution of normal lymphocytes by immune ones as responding cells in the "one-way" mixed lymphocyte culture (MLC) results in inhibition of the DNA synthesis. This phenomenon is reproduced irrespective of the mouse strains used, immunization method (in vivo or in vitro) and various MLC techniques (macro- or microcultures, the presence of mercaptoethanol in the medium, control variants). Unlike the cytotoxic effect of immune lymphocytes showing a sharp peak on the 8th day after in vivo immunization, inhibition of the DNA synthesis activation in MLC occurred as the second day, and persisted for several weeks after immunization; this inhibition was reproduced on addition of a small fraction (5 to 10%) of immune lymphocytes to normal ones.
在“单向”混合淋巴细胞培养（MLC）中，用免疫淋巴细胞替代正常淋巴细胞作为反应细胞会导致DNA合成受到抑制。无论所用小鼠品系、免疫方法（体内或体外）以及各种MLC技术（宏观或微量培养、培养基中巯基乙醇的存在、对照变体）如何，这种现象都会重现。与免疫淋巴细胞在体内免疫后第8天出现尖锐峰值的细胞毒性作用不同，MLC中DNA合成激活的抑制在免疫后第2天就出现，并在免疫后持续数周；当向正常淋巴细胞中添加一小部分（5%至10%）免疫淋巴细胞时，这种抑制作用会重现。
