碱性成纤维细胞生长因子和肝素对来自肝素黄杆菌的肝素/硫酸乙酰肝素降解酶的相反作用。

Opposite effects of basic fibroblast growth factor and heparin on heparin/heparan sulfate degrading enzymes from Flavobacterium heparinum.

作者信息

Cavari S, Vannucchi S

机构信息

Institute of General Pathology, University of Florence, Firenze, Italy.

出版信息

Biochem Mol Biol Int. 1996 Jul;39(4):823-31. doi: 10.1080/15216549600201921.

DOI:10.1080/15216549600201921

PMID:8843352

Abstract

Heparinase I is normally inhibited by an excess or substrate, in this paper we describe that bFGF counteracts the inhibiting effect of the substrate and that heparinase I activity is increased in the presence of bFGF. This effect was observed using heparin concentrations ranging from 10(-9) to 10(-7) M with either a ten fold molar excess or an equimolar concentration of bFGF. In addition, the degree of depolymerization of heparan sulfate produced by heparitinase from Flavobacterium heparinum was increased in the presence of bFGF.

摘要

肝素酶I通常会被过量的底物所抑制，在本文中我们描述了碱性成纤维细胞生长因子（bFGF）可抵消底物的抑制作用，并且在bFGF存在的情况下肝素酶I的活性会增加。使用浓度范围为10^(-9)至10^(-7)M的肝素，同时加入摩尔过量十倍或等摩尔浓度的bFGF时可观察到这种效应。此外，在bFGF存在的情况下，来自肝素黄杆菌的类肝素酶产生的硫酸乙酰肝素的解聚程度增加。