高效液相色谱法检测和测定[3H]阿糖胞苷的主要代谢产物
Detection and determination of the major metabolites of [3H]cytosine arabinoside by high-performance liquid chromatography.
作者信息
Braess J, Pförtner J, Kaufmann C C, Ramsauer B, Unterhalt M, Hiddemann W, Schleyer E
机构信息
Department of Hematology and Oncology, University of Göttingen, Germany.
出版信息
J Chromatogr B Biomed Appl. 1996 Feb 9;676(1):131-40. doi: 10.1016/0378-4347(95)00372-x.
An ion-pair high-performance liquid chromatographic assay involving solid-phase scintillation detection was established for the rapid identification and determination of all major metabolites of tritium-labelled cytosine arabinoside (Ara-C) in an in vitro system. In a single run of 50 min, Ara-C, Ara-CMP, Ara-CDP-choline, Ara-CDP, Ara-U, Ara-UMP, Ara-CTP, Ara-UDP and Ara-UTP can be measured. The method is fast, sensitive, with limits of detection ranging from 40 to 200 pg (absolute), and highly reproducible.
建立了一种涉及固相闪烁检测的离子对高效液相色谱分析法，用于在体外系统中快速鉴定和测定氚标记阿糖胞苷（Ara-C）的所有主要代谢物。在单次50分钟的运行中，可以测定Ara-C、Ara-CMP、Ara-CDP-胆碱、Ara-CDP、Ara-U、Ara-UMP、Ara-CTP、Ara-UDP和Ara-UTP。该方法快速、灵敏，检测限为40至200 pg（绝对），且重现性高。
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