Bon M C
CIRAD-Forêt/Innoprise, Corporation Tawau, Sabah, Malaysia.
Electrophoresis. 1996 Jul;17(7):1248-52. doi: 10.1002/elps.1150170713.
A combination of a modified Feret' (Silvae Genet. 1971, 20, 46-50) extraction buffer and two types of electrophoresis with acrylamide and starch gels were used to characterize allozymes in mature vegetative tissue of a commercially high value species of rattans (Calamus subinermis). From the analysis of allelic segregation from single maternal rattans and their offspring, genetic control of the 16 observed banding zones, which were consistently scorable, was assumed. Seventeen gene loci were identified. The percentage of polymorphic loci within Calamus subinermis was much higher (70.5%) than expected levels of genetic diversity for tropical woody and non-woody species. It is thought that the protocol described may be applied to the analysis of the genetic diversity of all the endangered Calamus species.
采用改良的费雷特（Silvae Genet. 1971, 20, 46 - 50）提取缓冲液与两种分别使用丙烯酰胺凝胶和淀粉凝胶的电泳方法相结合，对一种具有商业高价值的藤本植物（单叶省藤）成熟营养组织中的等位酶进行表征。通过对单个母本藤及其后代的等位基因分离分析，假定对16个可一致计分的观察条带区域进行遗传控制。共鉴定出17个基因位点。单叶省藤内多态性位点的百分比（70.5%）远高于热带木本和非木本物种预期的遗传多样性水平。据认为，所描述的方案可应用于所有濒危省藤物种的遗传多样性分析。
