重组人干扰素和小鼠干扰素对单纯疱疹病毒1型在小鼠细胞中复制的差异作用。

Differential effect of recombinant human and mouse interferons on replication of herpes simplex virus type 1 in mouse cells.

作者信息

Wintergerst U, Gangemi J D, Kern E R, Whitley R J, Chatterjee S

机构信息

Department of Pediatrics, University of Alaboma at Birmingham 35294, USA.

出版信息

Antiviral Res. 1996 Aug;32(1):27-34. doi: 10.1016/0166-3542(95)00975-2.

DOI:10.1016/0166-3542(95)00975-2

PMID:8863993

Abstract

Pretreatment of murine (BALB/3T3) cells with either murine or recombinant hybrid human B/D interferon (IFN) blocked the release of infectious herpes simplex virus type 1 (HSV-1) from treated cells. The block in replication was not due to an effect on attachment of HSV-1 to the target cells or to toxic effects of IFN. Immunoblot analyses showed that murine IFN significantly reduced the expression of virus-specific proteins in IFN-treated cells. In contrast, B/D IFN had no major effect on the expression of viral proteins in treated cells. In support of the above observation, electron microscopy of virus-infected cells displayed formation of nucleocapsids within the nucleus of IFN-treated cells. However, the expression of glycoproteins B and D was reduced in B/D IFN-treated cells. These results suggested that murine IFN blocked HSV-1 replication at an early stage whereas B/D IFN inhibited HSV-1 replication at a late stage in virus morphogenesis.

摘要

用鼠源或重组人源B/D干扰素（IFN）预处理鼠源（BALB/3T3）细胞，可阻止感染性单纯疱疹病毒1型（HSV-1）从处理过的细胞中释放。复制受阻并非由于对HSV-1与靶细胞的附着有影响，也不是IFN的毒性作用所致。免疫印迹分析表明，鼠源IFN显著降低了IFN处理细胞中病毒特异性蛋白的表达。相比之下，B/D IFN对处理细胞中病毒蛋白的表达没有主要影响。为支持上述观察结果，对病毒感染细胞进行电子显微镜检查显示，IFN处理细胞的细胞核内形成了核衣壳。然而，B/D IFN处理细胞中糖蛋白B和D的表达降低。这些结果表明，鼠源IFN在早期阶段阻断HSV-1复制，而B/D IFN在病毒形态发生的后期阶段抑制HSV-1复制。