Parkes H, Gould A R
CSIRO, Australian Animal Health Laboratory, Geelong, Australia.
Virus Res. 1996 Oct;44(2):111-22. doi: 10.1016/0168-1702(96)01344-5.
Sequence analyses of VP3 gene segments of Wongorr virus isolates from the Northern Territory of Australia were compared with the cognate gene segments from Picola and Paroo River viruses. Previous serological investigations had demonstrated some relationships between these viruses, however VP3 gene sequence and phylogenetic analyses placed these viruses within the same serogroup which was distinct from other described orbivirus serogroups. A polymerase chain reaction (PCR) was developed for the detection of this serogroup and used to identify and determine partial sequence data for other isolates of the virus. Wongorr virus and the other tick and mosquito-borne orbiviruses (Kemerovo and Corriparta), were more closely related than the Culicoides transmitted orbiviruses, such as bluetongue (BTV) and African horse sickness virus (AHSV) which were shown to be on a separate branch of the orbivirus phylogenetic tree.
对从澳大利亚北领地分离出的翁戈尔病毒（Wongorr virus）的VP3基因片段进行了序列分析，并与皮科拉病毒（Picola virus）和帕鲁河病毒（Paroo River virus）的同源基因片段进行了比较。先前的血清学研究已证明这些病毒之间存在一些关联，然而VP3基因序列和系统发育分析将这些病毒置于同一个血清群中，该血清群与其他已描述的环状病毒血清群不同。开发了一种聚合酶链反应（PCR）用于检测该血清群，并用于鉴定和确定该病毒其他分离株的部分序列数据。翁戈尔病毒与其他蜱传和蚊传环状病毒（克麦罗沃病毒（Kemerovo virus）和科里帕塔病毒（Corriparta virus））的关系比库蠓传播的环状病毒（如蓝舌病病毒（BTV）和非洲马瘟病毒（AHSV））更为密切，后两者在环状病毒系统发育树中位于单独的分支上。
