经羊痘化的牛瘟病毒在体外适应于在非洲绿猴肾细胞（Vero细胞）中生长。

Adaptation of caprinised rinderpest virus to grow in Vero cells in vitro.

作者信息

Parida M M, Bandyopadhyay S K

机构信息

Division of Virology, Indian Veterinary Research Insitute, Nainital (UP), India.

出版信息

Acta Virol. 1996 Feb;40(1):45-8.

PMID:8886098

Abstract

Caprinised rinderpest virus GTV strain (GTV) was adapted to grow in Vero cells (vGTV) by polyethylene glycol-mediated fusion of infected goat spleenocytes with non-infected Vero cells. The usual methods of infection of cell culture, i.e. virus adsorption or co-cultivation, were not successful. vGTV-induced cytopathic changes in Vero cells were similar to those reported for rinderpest virus. Virus titers increased with the passage number but the virulence for goats decreased. Immunoblot analysis did not reveal any difference between vGTV and vRBOK, the tissue culture rinderprest virus RBOK strain adapted to Vero cells.

摘要

通过聚乙二醇介导感染山羊脾细胞与未感染的非洲绿猴肾细胞融合，使羊痘化牛瘟病毒GTV株（GTV）适应在非洲绿猴肾细胞中生长（vGTV）。细胞培养常用的感染方法，即病毒吸附或共培养，均未成功。vGTV在非洲绿猴肾细胞中诱导的细胞病变效应与牛瘟病毒报道的相似。病毒滴度随传代次数增加，但对山羊的毒力下降。免疫印迹分析未显示vGTV与vRBOK（适应非洲绿猴肾细胞的组织培养牛瘟病毒RBOK株）之间有任何差异。