在肥大细胞中鉴定出白细胞介素-15/T的一种新型受体/信号转导途径。

Identification of a novel receptor/signal transduction pathway for IL-15/T in mast cells.

作者信息

Tagaya Y, Burton J D, Miyamoto Y, Waldmann T A

机构信息

Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1374, USA.

出版信息

EMBO J. 1996 Sep 16;15(18):4928-39.

Interleukin-15/T(IL-15) is a growth factor that utilizes IL-2 receptor (IL-2R) components in addition to its private binding protein IL-15R(alpha) in T-cells. Here, we report that IL-15 induces mast cell proliferation in the absence of IL-2R alpha and beta. Using transfectants of these cells with a cytoplasmic-truncated mutant of gamma(c), we demonstrated that IL-15 signaling in mast cells does not involve gamma(c). Cross-linking of mast cells with [(125)I]IL-15 revealed a 60-65 kDa IL-15 binding protein that is distinct from known components of T-cell IL-15 receptors. Mast cell IL-15 receptors recruit JAK-2 and STAT-5, instead of JAK1/3 and STAT3/5 that are activated in T-cells. Thus IL-15 is a mast cell growth factor that utilizes a novel receptor and distinct signaling pathway.

白细胞介素-15/T（IL-15）是一种生长因子，在T细胞中，它除了利用其特异性结合蛋白IL-15Rα外，还利用白细胞介素-2受体（IL-2R）成分。在此，我们报告IL-15在缺乏IL-2Rα和β的情况下可诱导肥大细胞增殖。通过用γ(c)的胞质截短突变体转染这些细胞，我们证明肥大细胞中的IL-15信号传导不涉及γ(c)。用[¹²⁵I]IL-15对肥大细胞进行交联，发现一种60 - 65 kDa的IL-15结合蛋白，它不同于T细胞IL-15受体的已知成分。肥大细胞IL-15受体募集JAK-2和STAT-5，而不是在T细胞中被激活的JAK1/3和STAT3/5。因此，IL-15是一种利用新型受体和独特信号通路的肥大细胞生长因子。