Saito M, Eto M, Okada M, Iwashima Y, Makino I
Second Dept of Internal Medicine, Asahikawa Medical College, Japan.
Artery. 1996;22(3):155-63.
We tested the ability of remnant-like particles (RLP) from NIDDM patients to stimulate cholesteryl ester synthesis in human monocyte-derived macrophages. Six NIDDM patients were studied together with 7 non-diabetic subjects. All had apolipoprotein (apo) E3/3 phenotype. RLP were isolated using an immunoaffinity gel mixture of anti apo B-100 and anti apo A-1 monoclonal antibodies coupled to Sepharose 4B. Plasma levels of triglyceride, total cholesterol (chol) and high density lipoprotein-chol were not statistically different, but plasma levels of RLP-chol were significantly (p < 0.05) higher in NIDDM patients (10.5 +/- 2.2 mg/dl) than in non-diabetic controls (5.0 +/- 1.7 mg/dl). The effects of RLP from NIDDM patients on macrophage cholesteryl ester synthesis were estimated. 14C-oleate incorporation into cholesteryl esters in macrophages was significantly (p < 0.01) higher in NIDDM patients with apo E3/3 (0.326 +/- 0.037 nmole/mg cell protein) than in non-diabetic controls with apo E3/3 (0.181 +/- 0.011 nmole/ mg cell protein). It is suggested that RLP from NIDDM play a role in the accumulation of cholesteryl esters and are one of the risk factors for the acceleration of atherosclerosis in NIDDM.
我们检测了非胰岛素依赖型糖尿病（NIDDM）患者的类残余颗粒（RLP）刺激人单核细胞衍生巨噬细胞合成胆固醇酯的能力。对6例NIDDM患者和7名非糖尿病受试者进行了研究。所有受试者均具有载脂蛋白（apo）E3/3表型。使用偶联到琼脂糖4B上的抗apo B - 100和抗apo A - 1单克隆抗体的免疫亲和凝胶混合物分离RLP。甘油三酯、总胆固醇（chol）和高密度脂蛋白胆固醇的血浆水平无统计学差异，但NIDDM患者的RLP - chol血浆水平（10.5±2.2mg/dl）显著高于非糖尿病对照组（5.0±1.7mg/dl，p<0.05）。评估了NIDDM患者的RLP对巨噬细胞胆固醇酯合成的影响。具有apo E3/3的NIDDM患者巨噬细胞中14C - 油酸掺入胆固醇酯的量（0.326±0.037nmol/mg细胞蛋白）显著高于具有apo E3/3的非糖尿病对照组（0.181±0.011nmol/mg细胞蛋白，p<0.01）。提示NIDDM患者的RLP在胆固醇酯积累中起作用，并且是NIDDM患者动脉粥样硬化加速的危险因素之一。
