Popovic T, Kombarova S Y, Reeves M W, Nakao H, Mazurova I K, Wharton M, Wachsmuth I K, Wenger J D
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Diseases Control and Prevention, Atlanta, GA 30333, USA.
J Infect Dis. 1996 Nov;174(5):1064-72. doi: 10.1093/infdis/174.5.1064.
The largest diphtheria outbreak in the developed world since the 1960s began in the Russian federation in 1990. One hundred fifty-six Corynebacterium diphtheriae strains from throughout Russia, selected for temporal and geographic diversity, were assayed by ribotyping and multilocus enzyme electrophoresis (MEE). These tests showed significant genetic diversity within the C. diphtheriae species, and ribotyping and MEE data generally correlated well with epidemiologic data. A distinct clonal group of C. diphtheriae isolates (ET 8 complex) emerged in Russia in 1990 as the current outbreak began, and as the outbreak has progressed, these organisms have made up increasingly larger proportions of the strains that are isolated. Furthermore, the main characteristic of the epidemic strains is a specific combination of ET 8 and ribotypes G1 and G4. This study confirms the epidemiologic utility of the molecular subtyping methods that detected the epidemic clone and addresses the clone's origin and relation to C. diphtheriae from throughout Russia.
20世纪60年代以来发达国家最大规模的白喉疫情于1990年在俄罗斯联邦爆发。从俄罗斯各地选取了156株具有时间和地理多样性的白喉棒状杆菌菌株，通过核糖体分型和多位点酶电泳（MEE）进行检测。这些测试表明白喉棒状杆菌物种内存在显著的遗传多样性，核糖体分型和MEE数据通常与流行病学数据相关性良好。1990年随着当前疫情的开始，俄罗斯出现了一个独特的白喉棒状杆菌分离株克隆群（ET 8复合体），并且随着疫情的发展，这些菌株在分离出的菌株中所占比例越来越大。此外，流行菌株的主要特征是ET 8与核糖体分型G1和G4的特定组合。本研究证实了检测流行克隆的分子分型方法在流行病学中的实用性，并探讨了该克隆的起源以及与俄罗斯各地白喉棒状杆菌的关系。
