环磷酸腺苷（cAMP）对头孢菌素诱导的猪肾细胞系LLC-PK1损伤的改善作用。

Amelioration by cAMP of cephaloridine-induced injury in the porcine kidney cell line LLC-PK1.

作者信息

Kawai Y, Gemba M

机构信息

Division of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.

出版信息

Jpn J Pharmacol. 1996 Sep;72(1):67-70. doi: 10.1254/jjp.72.67.

DOI:10.1254/jjp.72.67

PMID:8902601

Abstract

We investigated the effects of a phosphodiesterase inhibitor and dibutyryl cAMP (dBcAMP) on the cell injury induced by cephaloridine (CER) in an established renal epithelial cell LLC-PK1. CER increased the leakage of lactate dehydrogenase (LDH) from LLC-PK1 cells to the medium and the level of lipid peroxidation in the cells. 3-Isobutyl-1-methylxanthine, a phosphodiesterase inhibitor, increased cAMP content in LLC-PK1 cells and ameliorated the increase in LDH leakage induced by CER, dBcAMP reduced the cell injury induced by CER. Our results suggest that a signalling pathway of cAMP protects against CER-induced renal cell injury, which is probably due to generation of oxygen radicals.

摘要

我们研究了磷酸二酯酶抑制剂和二丁酰环磷腺苷（dBcAMP）对已建立的肾上皮细胞LLC-PK1中头孢菌素（CER）诱导的细胞损伤的影响。CER增加了乳酸脱氢酶（LDH）从LLC-PK1细胞向培养基中的泄漏以及细胞中脂质过氧化的水平。磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤增加了LLC-PK1细胞中的环磷腺苷含量，并改善了CER诱导的LDH泄漏增加，dBcAMP减轻了CER诱导的细胞损伤。我们的结果表明，环磷腺苷的信号通路可预防CER诱导的肾细胞损伤，这可能是由于氧自由基的产生。