Litteria M
Brain Res. 1977 Aug 26;132(2):287-99. doi: 10.1016/0006-8993(77)90422-x.
The purpose of the present study was to determine if the administration of testosterone propionate (TP) to neonatal rats is followed in vivo by alterations in the transport of the non-metabolizable amino acid, alpha-aminoisobutyric acid (AIB), into specific regions of the brain. Forty-eigh hours after birth, male and female rats were injected s.c. with either 1,25 mg TP or an equivalent volume of vehicle. Five, 10 and 17 days after birth, control and TP-treated rats were decapitated at intervals of 2, 5, 60 and 300 min after the i.p. injection of 0.25 muCi [1-14C]alpha-aminoisobutyric acid/g body weight. Twelve brain regions, i.e., amygdala, cerebellum, corpora quadrigemina, frontal cortex, hypothalamus, medulla, occipital cortex, olfactory bulbs, olfactory tubercles, parietal cortex, pons, pyriform cortex and samples of serum were analyzed in terms of disint./min/mg tissue and as tissue/serum (T/S) ratios. At the end of 300 min there was a significant increase in the active transport of AIB in all brain regions of the 5-days-old TP-treated rats. Similarly, by 300 min, the active transport of AIB was significantly increased in all brain regions sxcept cerebellum and pons of the 10-day-old TP-treated rats. The administration of TP to neonatal rats did not alter the accumulation and/or active transport of AIB in brain regions of the 17-day-old rat at any of the tested intervals. These data indicate that (1) neonatally administered TP enhanced (either directly or indirectly) the transport and/or accumuation of AIB in specific brain regions of 5- and 10-day-old rats and (2) the effectiveness of the steroid decreased with the age of the rat.
本研究的目的是确定给新生大鼠注射丙酸睾酮（TP）后，其体内不可代谢氨基酸α-氨基异丁酸（AIB）向脑特定区域的转运是否会发生改变。出生48小时后，对雄性和雌性大鼠皮下注射1.25毫克TP或等量的溶剂。出生后5天、10天和17天，在腹腔注射0.25微居里/克体重的[1-14C]α-氨基异丁酸后，每隔2、5、60和300分钟分别将对照大鼠和TP处理的大鼠断头。分析了12个脑区，即杏仁核、小脑、四叠体、额叶皮质、下丘脑、延髓、枕叶皮质、嗅球、嗅结节、顶叶皮质、脑桥、梨状皮质以及血清样本，测定其每分钟每毫克组织的衰变数以及组织/血清（T/S）比值。在300分钟结束时，5日龄TP处理大鼠的所有脑区中AIB的主动转运显著增加。同样，到300分钟时，10日龄TP处理大鼠除小脑和脑桥外的所有脑区中AIB的主动转运也显著增加。在任何测试时间间隔，给新生大鼠注射TP均未改变17日龄大鼠脑区中AIB的积累和/或主动转运。这些数据表明：（1）新生期给予TP可（直接或间接）增强5日龄和10日龄大鼠特定脑区中AIB的转运和/或积累；（2）该类固醇的作用效果随大鼠年龄增长而降低。
