嗜酸性粒细胞通过与纤连蛋白黏附对细胞死亡的调节。

Regulation of eosinophil cell death by adhesion to fibronectin.

作者信息

Higashimoto I, Chihara J, Kakazu T, Kawabata M, Nakajima S, Osame M

机构信息

Third Department of Internal Medicine, Kagoshima University Faculty of Medicine, Japan.

出版信息

Int Arch Allergy Immunol. 1996;111 Suppl 1:66-9. doi: 10.1159/000237420.

DOI:10.1159/000237420

PMID:8906118

Abstract

Fibronectin is an extracellular matrix (ECM) that binds to very late antigen-4 (a beta 1 integrin molecule) expressed by eosinophils. To investigate the effect of adherence to fibronectin on regulation of eosinophil cell death, survival of eosinophils was examined by trypan blue exclusion and Fas antigen expression on the cell surface using an eosinophilic cell line (EoL-1). Adhesion to fibronectin resulted in prolongation of eosinophil survival (fibronectin vs. bovine serum albumin (BSA), 62.9 +/- 5.10 vs. 53.9 +/- 4.30% viability at 72 h) and the decreasement of Fas antigen expression on EoL-1 (fibronectin vs. BSA, 24.3 +/- 2.15 vs. 74.5 +/- 8.25% positive). These findings suggest that eosinophil adhesion to ECM via adhesion molecules plays an important role in the pathogenesis of allergic inflammation, which involves eosinophil accumulation at the inflammatory site, through regulation of eosinophil cell death.

摘要

纤连蛋白是一种细胞外基质（ECM），它能与嗜酸性粒细胞表达的极晚期抗原-4（一种β1整合素分子）结合。为了研究黏附于纤连蛋白对嗜酸性粒细胞死亡调节的影响，利用嗜酸性细胞系（EoL-1），通过台盼蓝排斥法检测嗜酸性粒细胞的存活率，并检测细胞表面Fas抗原的表达。黏附于纤连蛋白可延长嗜酸性粒细胞的存活时间（纤连蛋白组与牛血清白蛋白（BSA）组相比，72小时时的存活率分别为62.9±5.10%和53.9±4.30%），并降低EoL-1细胞表面Fas抗原的表达（纤连蛋白组与BSA组相比，阳性率分别为24.3±2.15%和74.5±8.25%）。这些发现表明，嗜酸性粒细胞通过黏附分子与细胞外基质的黏附在变应性炎症的发病机制中起重要作用，变应性炎症涉及嗜酸性粒细胞在炎症部位的积聚，这一过程是通过调节嗜酸性粒细胞的细胞死亡实现的。