Kanoh H, Kai M, Wada I
Department of Biochemistry, Sapporo Medical University School of Medicine, Japan.
J Lipid Mediat Cell Signal. 1996 Sep;14(1-3):245-50. doi: 10.1016/0929-7855(96)00532-9.
Diacylglycerol and phosphatidate serve as key lipid intermediates of glycerolipid biosynthesis and also as the second messengers liberated in signal-stimulated cells. The molecular properties of enzymes involved in the metabolic processing of the two lipids are largely unknown. We have revealed by cDNA cloning the presence of a novel enzyme family of diacylglycerol kinase (DGK) having unique structural characteristics. Furthermore, we recently identified the plasma membrane-bound phosphatidate phosphatase (PAP) as a 35 kDa glycoprotein. Detailed studies on the two enzymes would contribute to understanding the molecular mechanisms underlying the control of both glycerolipid biosynthesis and signal transduction.
二酰基甘油和磷脂酸是甘油olipid生物合成的关键脂质中间体，也是信号刺激细胞中释放的第二信使。参与这两种脂质代谢加工的酶的分子特性在很大程度上尚不清楚。我们通过cDNA克隆揭示了一种具有独特结构特征的新型二酰基甘油激酶（DGK）酶家族的存在。此外，我们最近确定质膜结合的磷脂酸磷酸酶（PAP）是一种35 kDa的糖蛋白。对这两种酶的详细研究将有助于理解甘油olipid生物合成和信号转导控制背后的分子机制。
