Tettamanti G, Prinetti A, Bassi R, Viani P, Giussani P, Riboni L
Department of Medical Chemistry and Biochemistry, University of Milan, Italy.
J Lipid Mediat Cell Signal. 1996 Sep;14(1-3):263-75. doi: 10.1016/0929-7855(96)00535-4.
The involvement of ceramide in the differentiation of two neuroblastoma cell lines, Neuro2a and SH-SY5Y, and cerebellar granule cells in primary culture was investigated. The following results were obtained: (a) the cellular content of ceramide markedly increased with induced differentiation of Neuro2a cells (inducers: RA, FCS deprivation), SH-SY5Y cells (inducers: RA, PMA), and spontaneous differentiation of cerebellar granule cells; (b) all the investigated cells in the differentiated form displayed a higher ability to produce ceramide from exogenously administered [3H]Sph-SM and expressed a higher content of neutral sphingomyelinase and, in the case of cerebellar granule cells, also of acidic sphingomyelinase; (c) inhibition of ceramide biosynthesis by Fumonisin B1 blocked the process of differentiation in Neuro2a and cerebellar granule cells; and (d) treatments capable of enhancing ceramide level (administration of sphingosine or C2-Ceramide) induced differentiation in both Neuro2a and SH-SY5Y cells. The data obtained support the notion that ceramide plays a general biomodulatory role in neural cell differentiation.
研究了神经酰胺在两种神经母细胞瘤细胞系Neuro2a和SH - SY5Y以及原代培养的小脑颗粒细胞分化中的作用。得到以下结果：(a) 随着Neuro2a细胞（诱导剂：视黄酸、血清饥饿）、SH - SY5Y细胞（诱导剂：视黄酸、佛波酯）的诱导分化以及小脑颗粒细胞的自发分化，神经酰胺的细胞含量显著增加；(b) 所有分化形式的被研究细胞从外源性给予的[3H]鞘氨醇 - 鞘磷脂产生神经酰胺的能力更高，并且中性鞘磷脂酶含量更高，对于小脑颗粒细胞而言，酸性鞘磷脂酶含量也更高；(c) 伏马菌素B1对神经酰胺生物合成的抑制作用阻断了Neuro2a和小脑颗粒细胞的分化过程；(d) 能够提高神经酰胺水平的处理（给予鞘氨醇或C2 - 神经酰胺）在Neuro2a和SH - SY5Y细胞中均诱导分化。所获得的数据支持神经酰胺在神经细胞分化中起普遍生物调节作用这一观点。
