Korn W M, Oide Weghuis D E, Suijkerbuijk R F, Schmidt U, Otto T, du Manoir S, Geurts van Kessel A, Harstrick A, Seeber S, Becher R
West German Cancer Center, University of Essen, Medical School, Federal Republic of Germany.
Genes Chromosomes Cancer. 1996 Oct;17(2):78-87. doi: 10.1002/(SICI)1098-2264(199610)17:2<78::AID-GCC2>3.0.CO;2-Y.
To extend the results of conventional cytogenetic analysis of testicular germ cell tumors (TGCTs), we applied the new molecular cytogenetic method of comparative genomic hybridization (CGH), which enables the detection of chromosomal imbalances without the need for dividing cells. DNA from II TGCTs was studied by CGH. In all tumors examined, gain of 12p, mostly of the whole p arm, could be demonstrated. However, in three tumors, an amplification of 12p material restricted to the chromosomal bands 12p11.2-p12.1 was found. Further fluorescence in situ hybridization (FISH) analysis using a yeast artificial chromosome (YAC) that was previously mapped to that region revealed multiple copies of that chromosomal segment in interphase nuclei of these tumors. This finding is an important clue to the localization of candidate protooncogenes at 12p involved in TGCTs. Gains of small chromosomal regions at 2p, 4q, 6p, and 19p were also detected recurrently. Furthermore, gains of chromosomes 8, 14, 21, and X as well as loss of chromosome 13 were frequent findings. In conclusion, CGH provides new insights into genetic alterations of TGCTs. By using CGH, chromosomal subregions could be identified that may harbor genes involved in the pathogenesis of this malignancy.
为扩展睾丸生殖细胞肿瘤（TGCTs）传统细胞遗传学分析的结果，我们应用了比较基因组杂交（CGH）这种新的分子细胞遗传学方法，该方法能够在无需分裂细胞的情况下检测染色体失衡。通过CGH对11例TGCTs的DNA进行了研究。在所有检测的肿瘤中，均可证实存在12p的增益，多数为整个p臂的增益。然而，在3例肿瘤中，发现12p物质的扩增局限于染色体带12p11.2 - p12.1。使用先前定位到该区域的酵母人工染色体（YAC）进行的进一步荧光原位杂交（FISH）分析显示，这些肿瘤的间期核中该染色体片段存在多个拷贝。这一发现是定位参与TGCTs的12p候选原癌基因的重要线索。2p、4q、6p和19p小染色体区域的增益也经常被检测到。此外，染色体8、14、21和X的增益以及染色体13的缺失也是常见的发现。总之，CGH为TGCTs的基因改变提供了新的见解。通过使用CGH，可以识别可能含有参与这种恶性肿瘤发病机制的基因的染色体亚区域。
