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1
Effects of antisense DNA against the alpha-sarcin stem-loop structure of the ribosomal 23S rRNA.针对核糖体23S rRNA的α-肌动蛋白茎环结构的反义DNA的作用。
Nucleic Acids Res. 1996 Oct 15;24(20):3996-4002. doi: 10.1093/nar/24.20.3996.
2
Resistance of both the 5'- and 3'-domain of isolated Escherichia coli 23S rRNA against digestion with alpha-sarcin.分离的大肠杆菌23S rRNA的5'和3'结构域对α-肌动蛋白消化的抗性
Biochem Int. 1988 Oct;17(4):617-27.
3
Alpha-sarcin cleavage of ribosomal RNA is inhibited by the binding of elongation factor G or thiostrepton to the ribosome.延伸因子G或硫链丝菌素与核糖体的结合可抑制核糖体RNA的α-肌动蛋白切割。
Nucleic Acids Res. 1991 Apr 11;19(7):1657-60. doi: 10.1093/nar/19.7.1657.
4
Probing surface structure of sarcin domain on ribosomes of Escherichia coli by complementary oligo DNAs and ribosome-inactivating protein.利用互补寡聚脱氧核糖核酸和核糖体失活蛋白探测大肠杆菌核糖体上肌动蛋白结构域的表面结构
J Biomed Sci. 1999 Jul-Aug;6(4):277-84. doi: 10.1007/BF02253569.
5
The action of pokeweed antiviral protein and ricin A-chain on mutants in the alpha-sarcin loop of Escherichia coli 23S ribosomal RNA.商陆抗病毒蛋白和蓖麻毒素A链对大肠杆菌23S核糖体RNAα-肌动蛋白环突变体的作用。
J Mol Biol. 1995 Dec 15;254(5):848-55. doi: 10.1006/jmbi.1995.0660.
6
Cleavage of the sarcin-ricin loop of 23S rRNA differentially affects EF-G and EF-Tu binding.核糖体 23S rRNA 上的 sarcin-ricin 环的切割会对 EF-G 和 EF-Tu 的结合产生不同的影响。
Nucleic Acids Res. 2010 Jul;38(12):4108-19. doi: 10.1093/nar/gkq151. Epub 2010 Mar 9.
7
Blocking of the function of alpha-sarcin domain of 28S ribosomal RNA using the synthetic oligonucleotides as antisense DNA probes.使用合成寡核苷酸作为反义DNA探针阻断28S核糖体RNA的α-肌动蛋白结构域的功能。
Acta Biochim Pol. 1992;39(1):65-73.
8
Hybridization of antisense oligonucleotides with alpha-sarcin loop region of Escherichia coli 23S rRNA.反义寡核苷酸与大肠杆菌23S rRNA的α-肌动蛋白环区域的杂交。
Nucleosides Nucleotides Nucleic Acids. 2004 Oct;23(6-7):895-906. doi: 10.1081/NCN-200026038.
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alpha-Sarcin domain is a fragment of 23S and 26S rRNA strategic for ribosome function.
Acta Biochim Pol. 1993;40(1):12-6.
10
Characterization of in vitro and in vivo mutations in non-conserved nucleotides in the ribosomal RNA recognition domain for the ribotoxins ricin and sarcin and the translation elongation factors.核糖体毒素蓖麻毒素和帚曲霉素以及翻译延伸因子的核糖体RNA识别结构域中非保守核苷酸的体外和体内突变特征分析
J Mol Biol. 1999 Jan 15;285(2):567-80. doi: 10.1006/jmbi.1998.2337.

引用本文的文献

1
Structural Studies Reveal the Role of Helix 68 in the Elongation Step of Protein Biosynthesis.结构研究揭示了螺旋 68 在蛋白质生物合成延伸步骤中的作用。
mBio. 2022 Apr 26;13(2):e0030622. doi: 10.1128/mbio.00306-22. Epub 2022 Mar 29.
2
Hitting bacteria at the heart of the central dogma: sequence-specific inhibition.直击中心法则的核心：序列特异性抑制。
Microb Cell Fact. 2007 Aug 10;6:24. doi: 10.1186/1475-2859-6-24.
3
Targeting the A site RNA of the Escherichia coli ribosomal 30 S subunit by 2'-O-methyl oligoribonucleotides: a quantitative equilibrium dialysis binding assay and differential effects of aminoglycoside antibiotics.用2'-O-甲基寡核糖核苷酸靶向大肠杆菌核糖体30S亚基的A位点RNA：定量平衡透析结合测定及氨基糖苷类抗生素的差异效应
Biochem J. 2004 Oct 15;383(Pt 2):201-8. doi: 10.1042/BJ20040246.
4
Inhibition of translation and bacterial growth by peptide nucleic acid targeted to ribosomal RNA.靶向核糖体RNA的肽核酸对翻译和细菌生长的抑制作用
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2073-6. doi: 10.1073/pnas.95.5.2073.
5
Placement of the alpha-sarcin loop within the 50S subunit: evidence derived using a photolabile oligodeoxynucleotide probe.α-肌动蛋白环在50S亚基中的定位：使用光不稳定寡脱氧核苷酸探针获得的证据。
Nucleic Acids Res. 1997 Nov 15;25(22):4562-9. doi: 10.1093/nar/25.22.4562.

本文引用的文献

1
The conformation of the sarcin/ricin loop from 28S ribosomal RNA.来自28S核糖体RNA的肌动蛋白/蓖麻毒素环的构象。
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9581-5. doi: 10.1073/pnas.90.20.9581.
2
The ribosomal RNA identity elements for ricin and for alpha-sarcin: mutations in the putative CG pair that closes a GAGA tetraloop.蓖麻毒素和α-肌动蛋白的核糖体RNA识别元件：封闭GAGA四环的假定CG对中的突变。
Nucleic Acids Res. 1994 Feb 11;22(3):321-4. doi: 10.1093/nar/22.3.321.
3
The sarcin/ricin loop, a modular RNA.肌动蛋白/蓖麻毒素环，一种模块化RNA。
J Mol Biol. 1995 Mar 17;247(1):81-98. doi: 10.1006/jmbi.1994.0124.
4
The site of action of alpha-sarcin on eukaryotic ribosomes. The sequence at the alpha-sarcin cleavage site in 28 S ribosomal ribonucleic acid.α-肌动蛋白对真核生物核糖体的作用位点。28S核糖体核糖核酸中α-肌动蛋白切割位点的序列。
J Biol Chem. 1982 Aug 10;257(15):9054-60.
5
An enzymatic approach for localization of oligodeoxyribonucleotide binding sites on RNA. Application to studying rRNA topography.一种用于定位RNA上寡脱氧核糖核苷酸结合位点的酶学方法。应用于研究核糖体RNA拓扑结构。
FEBS Lett. 1981 Aug 31;131(2):253-6. doi: 10.1016/0014-5793(81)80378-x.
6
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
7
DNA hybridization electron microscopy: ribosomal RNA nucleotides 1392-1407 are exposed in the cleft of the small subunit.DNA杂交电子显微镜观察：核糖体RNA的核苷酸1392 - 1407暴露于小亚基的裂隙中。
Proc Natl Acad Sci U S A. 1986 Jan;83(2):275-9. doi: 10.1073/pnas.83.2.275.
8
Alpha-sarcin cleaves ribosomal RNA at the alpha-sarcin site in the absence of ribosomal proteins.在没有核糖体蛋白的情况下，α-肌动蛋白在α-肌动蛋白位点切割核糖体RNA。
Biochem Biophys Res Commun. 1988 Jul 15;154(1):404-10. doi: 10.1016/0006-291x(88)90700-0.
9
Evidence that the G2661 region of 23S rRNA is located at the ribosomal binding sites of both elongation factors.23S rRNA的G2661区域位于两种延伸因子的核糖体结合位点的证据。
Biochimie. 1987 Sep;69(9):911-23. doi: 10.1016/0300-9084(87)90225-2.
10
Parameters for the preparation of Escherichia coli ribosomes and ribosomal subunits active in tRNA binding.制备在tRNA结合中具有活性的大肠杆菌核糖体及核糖体亚基的参数。
Methods Enzymol. 1988;164:658-70. doi: 10.1016/s0076-6879(88)64076-6.

针对核糖体23S rRNA的α-肌动蛋白茎环结构的反义DNA的作用。

Effects of antisense DNA against the alpha-sarcin stem-loop structure of the ribosomal 23S rRNA.

作者信息

Meyer H A, Triana-Alonso F, Spahn C M, Twardowski T, Sobkiewicz A, Nierhaus K H

机构信息

Max-Planck-Institut für Molekulare Genetik, AG Ribosomen, Berlin, Germany.

出版信息

Nucleic Acids Res. 1996 Oct 15;24(20):3996-4002. doi: 10.1093/nar/24.20.3996.

DOI:10.1093/nar/24.20.3996
PMID:8918803
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC146203/
Abstract

Antisense DNAs complementary against various sequences of the alpha-sarcin domain (C2646-G2674) of 23S rRNA from Escherichia coli were hybridized to naked 23S rRNA as well as to 70S ribosomes. Saturation levels of up to 0.4 per 70S ribosome were found, the identical fraction was susceptible to the attack of the RNase alpha-sarcin. The hybridization was specific as demonstrated with RNase H digestion, sequencing the resulting fragments and blockage of the action of alpha-sarcin. The RNase alpha-sarcin seems to approach its cleavage site from the 3' half of the loop of the alpha-sarcin domain. Hybridization is efficiently achieved at 37 degrees C and can extend at least into the 3' strand of the stem of the alpha-sarcin domain. However, the inhibition of alpha-sarcin activity is observed at 30 degrees C but not at 37 degrees C. For a significant inhibition of poly(Phe) synthesis the temperature had to be lowered to 25 degrees C. The results imply that the alpha-sarcin domain changes its conformation during protein synthesis and that the conformational changes may include a melting of the stem of the alpha-sarcin domain.

摘要

与来自大肠杆菌的23S rRNA的α-肌动蛋白结构域（C2646 - G2674）的各种序列互补的反义DNA与裸露的23S rRNA以及70S核糖体杂交。发现每70S核糖体的饱和水平高达0.4，相同比例的部分易受RNaseα-肌动蛋白的攻击。如通过RNase H消化、对所得片段进行测序以及阻断α-肌动蛋白的作用所证明的，杂交是特异性的。RNaseα-肌动蛋白似乎从α-肌动蛋白结构域环的3'半部分接近其切割位点。在37℃下有效地实现杂交，并且可以至少延伸到α-肌动蛋白结构域茎的3'链中。然而，在30℃观察到α-肌动蛋白活性的抑制，但在37℃未观察到。为了显著抑制聚（苯丙氨酸）合成，温度必须降至25℃。结果表明，α-肌动蛋白结构域在蛋白质合成过程中改变其构象，并且构象变化可能包括α-肌动蛋白结构域茎的解链。