Ganbo T, Hisamatsu K, Inoue H, Mizukoshi A, Goto R, Murakami Y
Department of Otorhinolaryngology, Yamanashi Medical University, Japan.
Rhinology. 1995 Dec;33(4):199-202.
The effects of leukotrienes C4 and D4 on ciliary activity of human paranasal sinus mucosa were investigated in vitro. Normal mucosa was surgically obtained from human paranasal sinuses and incubated in the form of tissue culture. Ciliated cells were magnified under an inverted microscope, and ciliary activity was photoelectrically measured. LTD4 progressively inhibited ciliary activity, and showed a more potent effect on ciliary activity compared to LTC4. The concentrations of LTC4 and LTD4 in the incubation medium were determined by radioimmunoassay when the mucosa was incubated with 10(-8) M LTC4. The concentration of LTD4 gradually increased and after 90 min reached the maximum of 0.71 x 10(-8) M, while that of LTC4 was reduced to about 10% of its initial concentration within 60 min. These results suggested the possible conversion of LTC4 to LTD4 on the mucosa, and that LTC4 can inhibit ciliary activity by means of LTD4.
体外研究了白三烯C4和D4对人鼻旁窦黏膜纤毛活动的影响。正常黏膜通过手术取自人鼻旁窦，并以组织培养的形式进行孵育。在倒置显微镜下放大纤毛细胞，并通过光电测量纤毛活动。白三烯D4逐渐抑制纤毛活动，并且与白三烯C4相比，对纤毛活动显示出更强的作用。当黏膜与10(-8)M白三烯C4一起孵育时，通过放射免疫测定法测定孵育培养基中白三烯C4和白三烯D4的浓度。白三烯D4的浓度逐渐增加，90分钟后达到最大值0.71×10(-8)M，而白三烯C4的浓度在60分钟内降至其初始浓度的约10%。这些结果提示白三烯C4在黏膜上可能转化为白三烯D4，并且白三烯C4可通过白三烯D4抑制纤毛活动。
Zotero 插件