Hoshi F, Nagai D, Higuchi S, Noso T, Takahashi A, Kawamura S
Department of Veterinary Internal Medicine, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Vet Immunol Immunopathol. 1996 Sep;53(1-2):29-38. doi: 10.1016/0165-2427(96)05559-6.
Bovine beta 2-microglobulin (beta 2-m) was purified from colostrum milk in two chromatographic steps: anion-exchange chromatography, and gel filtration. The amino acid sequence was determined to confirm that the purified protein was beta 2-m. A molecular weight of 11.8 kDa beta 2-m was estimated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Bovine beta 2-m consists of 98 amino acid residues and contains one disulfide linkage connecting residues 25 and 79. The amino acid sequence determined by this study is different from a previously published sequence at three sites, but agrees with the amino acid sequence from cDNA. Thus, we can conclude that the amino acid sequence determined in this study is correct.
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