唐氏综合征和18三体妊娠孕妇血清中的热稳定且具有免疫反应性的胎盘碱性磷酸酶

Heat-stable and immunoreactive placental alkaline phosphatase in maternal serum from Down's syndrome and trisomy 18 pregnancies.

作者信息

Aitken D A, Syvertsen B S, Crossley J A, Berry E, Connor J M

机构信息

Duncan Guthrie Institute of Medical Genetics, Yorkhill, Glasgow, U.K.

出版信息

Prenat Diagn. 1996 Nov;16(11):1051-4. doi: 10.1002/(SICI)1097-0223(199611)16:11<1051::AID-PD988>3.0.CO;2-T.

DOI:10.1002/(SICI)1097-0223(199611)16:11<1051::AID-PD988>3.0.CO;2-T

PMID:8953641

Abstract

Placental alkaline phosphatase (ALP) activity was investigated in second-trimester maternal sera from 37 pregnancies with Down's syndrome, 28 pregnancies with trisomy 18, and in a series of 497 controls using a fluorimetric heat inactivation assay and specific immunoassay. After conversion of individual analyte values to multiples of the normal gestational median (MOM), no significant differences in total or placental ALP activities were found in the trisomy 21 or trisomy 18 cases (P > 0.01). In the Down's syndrome pregnancies, total ALP activity was 0.93 MOM, heat-stable ALP activity was 1.09 MOM, and placental ALP (by immunoassay) 0.96 MOM. In the trisomy 18 cases, total ALP activity was 0.90 MOM, heat-stable ALP activity was 0.79 MOM, and placental ALP (by immunoassay) 0.94 MOM. We conclude that neither total nor placental ALP activity is a useful marker for Down's syndrome or trisomy 18 screening.

摘要

采用荧光热灭活测定法和特异性免疫测定法，对37例唐氏综合征妊娠、28例18三体妊娠的孕中期母体血清以及497例对照进行胎盘碱性磷酸酶（ALP）活性研究。将个体分析物值换算为正常妊娠中位数的倍数（MOM）后，21三体或18三体病例的总ALP活性或胎盘ALP活性均无显著差异（P>0.01）。唐氏综合征妊娠中，总ALP活性为0.93MOM，热稳定ALP活性为1.09MOM，胎盘ALP（通过免疫测定）为0.96MOM。18三体病例中，总ALP活性为0.90MOM，热稳定ALP活性为0.79MOM，胎盘ALP（通过免疫测定）为0.94MOM。我们得出结论，总ALP活性和胎盘ALP活性均不是唐氏综合征或18三体筛查的有用标志物。