Mann K, Eckerskorn C, Mehrabian Z, Nalbandyan R M
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Biochem Mol Biol Int. 1996 Nov;40(5):881-7. doi: 10.1080/15216549600201493.
We describe the amino acid sequence of plantacyanin isolated from spinach leaves. This small basic copper-binding plant cupredoxin contains 91 amino acids (accession no. S70746) and its theoretical average mass of 9788.2 Da was confirmed by electrospray ionization mass spectrometry. When compared to the sequences of other plant cupredoxins, the most similar homologs were cucumber basic blue protein and stellacyanin from Laquer tree with 44-58% identical residues. Other known plant cupredoxins were significantly less similar which may allow to divide the plant cupredoxins into two subgroups, the single-domain cupredoxins (plantacyanin, cucumber basic blue protein and stellacyanin) and the two-domain, hydroxyproline-containing cupredoxins (cucumber peeling cupredoxin, basic blue protein from Arabidopsis thaliana and horseradish umecyanin).
我们描述了从菠菜叶中分离出的植蓝素的氨基酸序列。这种小型碱性铜结合植物铜蓝蛋白含有91个氨基酸（登录号：S70746），通过电喷雾电离质谱法证实其理论平均分子量为9788.2道尔顿。与其他植物铜蓝蛋白的序列相比，最相似的同源物是黄瓜碱性蓝蛋白和漆树中的紫树蓝蛋白，其相同残基为44 - 58%。其他已知的植物铜蓝蛋白相似度明显较低，这可能有助于将植物铜蓝蛋白分为两个亚组，即单结构域铜蓝蛋白（植蓝素、黄瓜碱性蓝蛋白和紫树蓝蛋白）和含羟脯氨酸的双结构域铜蓝蛋白（黄瓜皮铜蓝蛋白、拟南芥碱性蓝蛋白和辣根伞形蓝蛋白）。
