Shi Z Y, Liu P Y, Lau Y J, Lin Y H, HU B S
Section of Infectious Diseases, Taichung Veterans General Hospital, Taiwan, Republic of China.
J Clin Microbiol. 1997 Jan;35(1):325-7. doi: 10.1128/jcm.35.1.325-327.1997.
Pulsed-field gel electrophoresis (PFGE) typing was applied to the epidemiological investigation of 20 Serratia marcescens isolates collected from urine specimens of 17 patients and three urinals over a 2-month period. Twenty-five epidemiologically unrelated strains were also tested to determine the discriminatory power of PFGE. The PFGE fingerprints of each isolate were consistent in three different tests. The 20 outbreak isolates had an identical PFGE fingerprint pattern, while the epidemiologically unrelated strains demonstrated unique PFGE fingerprint patterns. The source of the outbreak was inadequately disinfected urinals. We conclude that PFGE served as a highly discriminatory and reproducible method for the epidemiological investigation of the outbreak of S. marcescens infection addressed by this study.
脉冲场凝胶电泳(PFGE)分型技术被应用于对在两个月内从17名患者的尿液标本和三个小便器中分离出的20株粘质沙雷氏菌进行流行病学调查。还对25株流行病学上无关的菌株进行了检测,以确定PFGE的鉴别能力。每个分离株的PFGE指纹图谱在三次不同的测试中是一致的。20株暴发分离株具有相同的PFGE指纹图谱模式,而流行病学上无关的菌株则显示出独特的PFGE指纹图谱模式。暴发的源头是未充分消毒的小便器。我们得出结论,PFGE是本研究中用于粘质沙雷氏菌感染暴发流行病学调查的一种高度鉴别性和可重复性的方法。