使用脉冲场凝胶电泳技术调查粘质沙雷氏菌的一次暴发。
Use of pulsed-field gel electrophoresis to investigate an outbreak of Serratia marcescens.
作者信息
Shi Z Y, Liu P Y, Lau Y J, Lin Y H, HU B S
机构信息
Section of Infectious Diseases, Taichung Veterans General Hospital, Taiwan, Republic of China.
出版信息
J Clin Microbiol. 1997 Jan;35(1):325-7. doi: 10.1128/jcm.35.1.325-327.1997.
Abstract
Pulsed-field gel electrophoresis (PFGE) typing was applied to the epidemiological investigation of 20 Serratia marcescens isolates collected from urine specimens of 17 patients and three urinals over a 2-month period. Twenty-five epidemiologically unrelated strains were also tested to determine the discriminatory power of PFGE. The PFGE fingerprints of each isolate were consistent in three different tests. The 20 outbreak isolates had an identical PFGE fingerprint pattern, while the epidemiologically unrelated strains demonstrated unique PFGE fingerprint patterns. The source of the outbreak was inadequately disinfected urinals. We conclude that PFGE served as a highly discriminatory and reproducible method for the epidemiological investigation of the outbreak of S. marcescens infection addressed by this study.
摘要
脉冲场凝胶电泳（PFGE）分型技术被应用于对在两个月内从17名患者的尿液标本和三个小便器中分离出的20株粘质沙雷氏菌进行流行病学调查。还对25株流行病学上无关的菌株进行了检测，以确定PFGE的鉴别能力。每个分离株的PFGE指纹图谱在三次不同的测试中是一致的。20株暴发分离株具有相同的PFGE指纹图谱模式，而流行病学上无关的菌株则显示出独特的PFGE指纹图谱模式。暴发的源头是未充分消毒的小便器。我们得出结论，PFGE是本研究中用于粘质沙雷氏菌感染暴发流行病学调查的一种高度鉴别性和可重复性的方法。
相似文献
1
Use of pulsed-field gel electrophoresis to investigate an outbreak of Serratia marcescens.使用脉冲场凝胶电泳技术调查粘质沙雷氏菌的一次暴发。
J Clin Microbiol. 1997 Jan;35(1):325-7. doi: 10.1128/jcm.35.1.325-327.1997.
2
Comparative evaluation of an automated repetitive-sequence-based PCR instrument versus pulsed-field gel electrophoresis in the setting of a Serratia marcescens nosocomial infection outbreak.自动化重复序列基 PCR 仪器与脉冲场凝胶电泳在医院感染爆发的黏质沙雷氏菌中的比较评估。
J Clin Microbiol. 2010 May;48(5):1690-5. doi: 10.1128/JCM.01528-09. Epub 2010 Mar 17.
3
4
Use of pulsed-field gel electrophoresis to investigate an outbreak of Serratia marcescens infection in a neonatal intensive care unit.运用脉冲场凝胶电泳技术调查新生儿重症监护病房中粘质沙雷氏菌感染的暴发情况。
J Hosp Infect. 2001 May;48(1):13-9. doi: 10.1053/jhin.2001.0947.
5
Interpretation of band differences to distinguish strains of Serratia marcescens by pulsed-field gel electrophoresis of XbaI DNA digests.通过XbaI酶切DNA的脉冲场凝胶电泳分析区分粘质沙雷氏菌菌株的条带差异解读
Epidemiol Infect. 2000 Aug;125(1):63-70. doi: 10.1017/s0950268899004112.
6
Use of pulsed-field gel electrophoresis typing to study an outbreak of infection due to Serratia marcescens in a neonatal intensive care unit.运用脉冲场凝胶电泳分型技术研究新生儿重症监护病房中由粘质沙雷氏菌引起的感染暴发。
J Clin Microbiol. 1996 Dec;34(12):3138-41. doi: 10.1128/jcm.34.12.3138-3141.1996.
7
Molecular epidemiology of an outbreak of Serratia marcescens in a neonatal intensive care unit.新生儿重症监护病房中粘质沙雷氏菌暴发的分子流行病学
Infect Control Hosp Epidemiol. 2001 Oct;22(10):630-4. doi: 10.1086/501834.
8
Nosocomial neonatal outbreak of Serratia marcescens--analysis of pathogens by pulsed field gel electrophoresis and polymerase chain reaction.医院内新生儿粘质沙雷氏菌暴发——通过脉冲场凝胶电泳和聚合酶链反应进行病原体分析
Infection. 2002 Oct;30(5):277-81. doi: 10.1007/s15010-002-2141-y.
9
Serratia marcescens Outbreak in a Neonatal Intensive Care Unit: New Insights from Next-Generation Sequencing Applications.黏质沙雷氏菌在新生儿重症监护病房的爆发：下一代测序应用的新见解。
J Clin Microbiol. 2018 Aug 27;56(9). doi: 10.1128/JCM.00235-18. Print 2018 Sep.
10
Outbreak of multidrug-resistant Serratia marcescens infection in a neonatal intensive care unit.新生儿重症监护病房多药耐药粘质沙雷菌感染暴发
Infect Control Hosp Epidemiol. 2008 May;29(5):418-23. doi: 10.1086/587969.
引用本文的文献
1
Cultivation and sequencing-free protocol for detection and typing.用于检测和分型的无需培养和测序的方案。
iScience. 2024 Mar 1;27(4):109402. doi: 10.1016/j.isci.2024.109402. eCollection 2024 Apr 19.
2
Genomic characterisation of an entomopathogenic strain of Serratia ureilytica in the critically endangered phasmid Dryococelus australis.在极度濒危的叶䗛属Dryococelus australis 中，对一种昆虫病原性粘质沙雷氏菌的基因组特征进行了研究。
PLoS One. 2022 Apr 20;17(4):e0265967. doi: 10.1371/journal.pone.0265967. eCollection 2022.
3
A Possible Outbreak by : Genetic Relatedness between Clinical and Environmental Strains.可能由临床和环境菌株的遗传相关性引起的爆发。
Int J Environ Res Public Health. 2021 Sep 17;18(18):9814. doi: 10.3390/ijerph18189814.
4
colonization in preterm neonates during their neonatal intensive care unit stay.早产儿在新生儿重症监护病房期间的定植。
Antimicrob Resist Infect Control. 2019 Aug 9;8:135. doi: 10.1186/s13756-019-0584-5. eCollection 2019.
5
High third-generation cephalosporin resistant Enterobacteriaceae prevalence rate among neonatal infections in Dakar, Senegal.塞内加尔达喀尔新生儿感染中第三代头孢菌素耐药肠杆菌科细菌的高流行率。
BMC Infect Dis. 2016 Oct 20;16(1):587. doi: 10.1186/s12879-016-1935-y.
6
Serratia infections: from military experiments to current practice.沙雷氏菌感染：从军事实验到当前实践。
Clin Microbiol Rev. 2011 Oct;24(4):755-91. doi: 10.1128/CMR.00017-11.
7
Human pathogen shown to cause disease in the threatened eklhorn coral Acropora palmata.已证实人类病原体可导致受威胁的鹿角珊瑚 Acropora palmata 患病。
PLoS One. 2011;6(8):e23468. doi: 10.1371/journal.pone.0023468. Epub 2011 Aug 17.
8
Comparative evaluation of an automated repetitive-sequence-based PCR instrument versus pulsed-field gel electrophoresis in the setting of a Serratia marcescens nosocomial infection outbreak.自动化重复序列基 PCR 仪器与脉冲场凝胶电泳在医院感染爆发的黏质沙雷氏菌中的比较评估。
J Clin Microbiol. 2010 May;48(5):1690-5. doi: 10.1128/JCM.01528-09. Epub 2010 Mar 17.
9
Application of molecular techniques to the study of hospital infection.分子技术在医院感染研究中的应用。
Clin Microbiol Rev. 2006 Jul;19(3):512-30. doi: 10.1128/CMR.00025-05.
10
Extended epidemic of nosocomial urinary tract infections caused by Serratia marcescens.由粘质沙雷氏菌引起的医院获得性尿路感染的广泛流行。
J Clin Microbiol. 2003 Oct;41(10):4726-32. doi: 10.1128/JCM.41.10.4726-4732.2003.
本文引用的文献
1
Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping.用于研究粘质沙雷氏菌分子流行病学的核糖体分型：与生物分型的比较。
J Clin Microbiol. 1995 Oct;33(10):2637-42. doi: 10.1128/jcm.33.10.2637-2642.1995.
2
Epidemiological survey of an outbreak of multiresistant Serratia marcescens by PCR-fingerprinting.采用聚合酶链反应指纹图谱技术对多重耐药粘质沙雷氏菌暴发流行进行的流行病学调查。
Infection. 1995 Sep-Oct;23(5):267-71. doi: 10.1007/BF01716283.
3
Variability of the random amplified polymorphic DNA assay among thermal cyclers, and effects of primer and DNA concentration.热循环仪之间随机扩增多态性DNA分析的变异性以及引物和DNA浓度的影响。
Mol Cell Probes. 1993 Aug;7(4):293-9. doi: 10.1006/mcpr.1993.1043.
4
Comparison of serotype, biotype and bacteriocin type with rDNA RFLP patterns for the type identification of Serratia marcescens.用于粘质沙雷氏菌分型鉴定的血清型、生物型、细菌素型与rDNA RFLP模式的比较
Epidemiol Infect. 1993 Aug;111(1):99-107. doi: 10.1017/s0950268800056727.
5
Nosocomial transmission of Serratia odorifera biogroup. 2: Case report demonstration by macrorestriction analysis of chromosomal DNA using pulsed-field gel electrophoresis.
Infect Control Hosp Epidemiol. 1994 Jun;15(6):390-3. doi: 10.1086/646936.
6
Comparison of four genotyping assays for epidemiological study of methicillin-resistant Staphylococcus aureus.
Eur J Clin Microbiol Infect Dis. 1994 May;13(5):420-4. doi: 10.1007/BF01972002.
7
Use of PCR to study epidemiology of Serratia marcescens isolates in nosocomial infection.运用聚合酶链反应（PCR）研究医院感染中粘质沙雷氏菌分离株的流行病学。
J Clin Microbiol. 1994 Aug;32(8):1935-8. doi: 10.1128/jcm.32.8.1935-1938.1994.
8
Comparative study of five different DNA fingerprint techniques for molecular typing of Streptococcus pneumoniae strains.用于肺炎链球菌菌株分子分型的五种不同DNA指纹技术的比较研究
J Clin Microbiol. 1995 Jun;33(6):1606-12. doi: 10.1128/jcm.33.6.1606-1612.1995.
9
Analysis of clonal relationships among isolates of Shigella sonnei by different molecular typing methods.采用不同分子分型方法分析宋内志贺菌分离株之间的克隆关系。
J Clin Microbiol. 1995 Jul;33(7):1779-83. doi: 10.1128/jcm.33.7.1779-1783.1995.
10
Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.解读脉冲场凝胶电泳产生的染色体DNA限制性图谱：细菌菌株分型标准
J Clin Microbiol. 1995 Sep;33(9):2233-9. doi: 10.1128/jcm.33.9.2233-2239.1995.
Zotero 插件