Veelaert D, Passier P, Devreese B, Vanden Broeck J, Van Beeumen J, Vullings H G, Diederen J H, Schoofs L, De Loof A
Laboratory for Developmental Physiology and Molecular Biology, Zoological Institute K. U. Leuven, Belgium.
Endocrinology. 1997 Jan;138(1):138-42. doi: 10.1210/endo.138.1.4855.
A methanolic extract of 7000 desert locust (Schistocerca gregaria) brains contains several factors that stimulate the in vitro release of adipokinetic hormone (AKH) by glandular cells of locust (Locusta migratoria and Schistocerca gregaria) corpora cardiaca. The most potent one has now been fully identified. Matrix-assisted laser desorption ionization mass spectrometry-time of flight analysis revealed a mass of 954.6 Da. The primary structure of the peptide, Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2, appeared identical to that of a previously identified crustacean cardioactive peptide. This myotropin was first isolated from the shore crab, Carcinus maenas, and later from several insect species, but was never reported in the context of AKH release. The present study shows that synthetic crustacean cardioactive peptide induces the release of AKH from corpora cardiaca in a dose-dependent manner when tested in concentrations ranging from 10(-5)-10(-9) M. This is the first demonstration in invertebrates of a peptide neurohormone controlling the release of a second peptide hormone.
7000只沙漠蝗虫（沙漠飞蝗）大脑的甲醇提取物中含有多种能刺激蝗虫（飞蝗和沙漠飞蝗）心侧体腺细胞体外释放脂肪动激素（AKH）的因子。目前，最有效的一种因子已被完全鉴定出来。基质辅助激光解吸电离飞行时间分析显示其分子量为954.6道尔顿。该肽的一级结构为Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2，与先前鉴定的一种甲壳类心脏活性肽相同。这种促肌动素最初是从滨蟹中分离出来的，后来在几种昆虫物种中也有发现，但从未在AKH释放的背景下被报道过。本研究表明，当以10(-5)-10(-9)M的浓度进行测试时，合成的甲壳类心脏活性肽能以剂量依赖的方式诱导心侧体释放AKH。这是在无脊椎动物中首次证明一种肽神经激素控制另一种肽激素的释放。
