Buc-Caron M H
Département de Biologie Moléculaire et ERS 67 du CNRS, Institut Pasteur, 25 rue du Docteur Roux, Paris Cedex 15, 75724, France.
Neurobiol Dis. 1995 Feb;2(1):37-47. doi: 10.1006/nbdi.1995.0004.
Putative neuroepithelial cells were explanted from several germinative zones of the brain of 5-to-12 week old human fetuses, obtained from legal abortions. In each case, an homogeneous population of neuroepithelial-like cells expressing nestin and vimentin was obtained. Cells proliferated in vitro in response to bFGF which favoured a neuroblastic differentiation with expression of MAP-5 and beta3-tubulin. Cells could be maintained and propagated as neuroblasts in serum-free medium. The multipotentiality of the cells was revealed by growing them in serum-containing medium, where a mixed population of cells of the neuronal lineage and of glial cells including putative radial glia, astrocytes and oligodendrocytes, progressively differentiated. These human progenitor cells proliferating in vitro have many potential applications in gene therapy of neurodegenerative diseases.
假定的神经上皮细胞取自5至12周大的人类胎儿大脑的几个生发区,这些胎儿是通过合法堕胎获得的。在每种情况下,都获得了表达巢蛋白和波形蛋白的神经上皮样细胞的同质群体。细胞在体外对碱性成纤维细胞生长因子(bFGF)有增殖反应,bFGF有利于神经母细胞分化并表达微管相关蛋白5(MAP-5)和β3-微管蛋白。细胞可以在无血清培养基中作为神经母细胞维持和增殖。通过在含血清培养基中培养这些细胞,揭示了它们的多能性,在这种培养基中,包括假定的放射状胶质细胞、星形胶质细胞和少突胶质细胞在内的神经元谱系细胞和神经胶质细胞的混合群体逐渐分化。这些在体外增殖的人类祖细胞在神经退行性疾病的基因治疗中有许多潜在应用。