Ibrahim S O, Johannessen A C, Idris A M, Hirsch J M, Vasstrand E N, Magnusson B, Nilsen R
Department of Oral Pathology, Haukeland Hospital, Bergen-Norway.
Int J Cancer. 1996 Dec 11;68(6):749-53. doi: 10.1002/(SICI)1097-0215(19961211)68:6<749::AID-IJC10>3.0.CO;2-W.
Immunohistochemistry was used to examine the expression of p53 in pre-malignant oral lesions and oral squamous-cell carcinomas (SCCs) from Swedish and Sudanese snuff-dippers, as well as in pre-malignant oral lesions and oral SCCs from non-snuff-dippers from the Sudan, Sweden and Norway. Of the 14 SCCs from Sudanese snuff-dippers, 21% (3/14) expressed p53. Of the 14, 60 and 41 SCCs from non-snuff-dippers from the Sudan, Sweden and Norway, 64% (9/14), 65% (39/60) and 68% (28/41) expressed p53, respectively. A statistically significant difference in expression of p53 was found in SCCs from Sudanese snuff-dippers compared to those from non-snuff-dippers from all/or any of the 3 countries. None of the suspected pre-malignant oral lesions from Sudanese snuff dippers or non-snuff-dippers expressed p53. Only 2 out of the 15 oral fibro-epithelial hyperplastic lesions from Swedish snuff-dippers expressed p53. Some of the oral epithelial dysplastic lesions, as well as the carcinoma in situ lesions from Norwegian non-snuff-dippers, expressed p53, while the oral fibro-epithelial hyperplastic lesions did not. The low relative frequency of p53 expression found in oral SCCs from snuff-dippers compared to those from non-snuff-dippers might suggest differences in mechanisms of oncogenic action induced by snuff. Alternatively, the pathogenesis of malignant oral lesions from snuff-dippers may follow a p53-independent pathway. In view of the unusually high levels of the tobacco-specific nitrosamines (TSNA) found in the type of snuff used in the Sudan, investigations of p53 mutations or oncogenes are needed.
采用免疫组织化学方法检测瑞典和苏丹鼻烟使用者的口腔癌前病变及口腔鳞状细胞癌（SCC）中p53的表达情况，同时检测苏丹、瑞典和挪威非鼻烟使用者的口腔癌前病变及口腔SCC中p53的表达情况。在苏丹鼻烟使用者的14例SCC中，21%（3/14）表达p53。在苏丹、瑞典和挪威非鼻烟使用者的14例、60例和41例SCC中，分别有64%（9/14）、65%（39/60）和68%（28/41）表达p53。与来自这3个国家中所有/或任何一个国家的非鼻烟使用者的SCC相比，苏丹鼻烟使用者的SCC中p53表达存在统计学显著差异。苏丹鼻烟使用者或非鼻烟使用者的可疑口腔癌前病变均未表达p53。瑞典鼻烟使用者的15例口腔纤维上皮增生性病变中只有2例表达p53。挪威非鼻烟使用者的一些口腔上皮发育异常病变以及原位癌病变表达p53，而口腔纤维上皮增生性病变未表达。与非鼻烟使用者相比，鼻烟使用者口腔SCC中p53表达的相对频率较低，这可能表明鼻烟诱导的致癌作用机制存在差异。或者，鼻烟使用者口腔恶性病变的发病机制可能遵循一条不依赖p53的途径。鉴于苏丹使用的鼻烟中发现的烟草特异性亚硝胺（TSNA）水平异常高，需要对p53突变或癌基因进行研究。
