鱼腥藻7120菌株的氮剥夺引发了一个基因簇的快速激活，该基因簇对于硝酸盐的吸收和利用至关重要。
Nitrogen deprivation of Anabaena sp. strain PCC 7120 elicits rapid activation of a gene cluster that is essential for uptake and utilization of nitrate.
作者信息
Cai Y, Wolk C P
机构信息
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824-1312, USA.
出版信息
J Bacteriol. 1997 Jan;179(1):258-66. doi: 10.1128/jb.179.1.258-266.1997.
Abstract
A transposon bearing luxAB, encoding luciferase, as a reporter of transcription was used to identify genes that are activated rapidly upon deprivation of Anabaena sp. strain PCC 7120 of fixed nitrogen. The three transposon-marked loci that were identified as responding most rapidly and strongly are closely linked and situated within nirA and nrtC and between nrtD and narB, genes whose products are responsible for uptake and reduction of NO2- and NO3-. A strain bearing a transcriptional fusion of narB to luxAB was constructed. Luminescence catalyzed by LuxAB was used to report on the expression of the interrupted genes. Whether these genes are regulated only coordinately is discussed.
摘要
携带luxAB（编码荧光素酶）的转座子作为转录报告基因，用于鉴定鱼腥藻PCC 7120菌株在固定氮缺乏时迅速被激活的基因。被鉴定为响应最迅速且强烈的三个转座子标记位点紧密相连，位于nirA和nrtC之间以及nrtD和narB之间，这些基因的产物负责NO2-和NO3-的摄取和还原。构建了一个携带narB与luxAB转录融合体的菌株。利用LuxAB催化的发光来报告中断基因的表达。讨论了这些基因是否仅协同调节。
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