人胃癌组织中免疫抑制细胞因子（IL-10、转化生长因子-β）基因表达
Immunosuppressive cytokines (IL-10, TGF-beta) genes expression in human gastric carcinoma tissues.
作者信息
Morisaki T, Katano M, Ikubo A, Anan K, Nakamura M, Nakamura K, Sato H, Tanaka M, Torisu M
机构信息
First Department of Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
出版信息
J Surg Oncol. 1996 Dec;63(4):234-9. doi: 10.1002/(SICI)1096-9098(199612)63:4<234::AID-JSO4>3.0.CO;2-D.
BACKGROUND
Contribution of immunosuppressive cytokines to tumor progression in many types of cancers has been suggested. To characterize the in vivo expression of immunosuppressive cytokines in gastric cancer, we analyzed the messenger RNA (mRNA) expression of transforming growth factor-beta (TGF-beta) and interleukin-10 (IL-10) in human gastric carcinoma tissues.
METHODS
Both tumor tissues and nontumor tissues from each resected specimen of 29 primary gastric carcinomas were tested for IL-10 and TGF-beta mRNA expression by the reverse transcriptase-polymerase chain reaction (RT-PCR), and the mRNA expression was correlated with various pathological parameters of the tumors.
RESULTS
Among the 29 tumors, mRNAs of TGF-beta and IL-10 were detected in 79% and 62% of tumor samples, respectively. These cytokines were detected only in 31% for TGF-beta and 17% for IL-10 in nontumor samples. Both mRNAs were frequently expressed in the poorly differentiated adenocarcinomas and the tumor tissues with high degree of stage or lymph node metastasis.
CONCLUSIONS
Local expression of immunosuppressive cytokines may contribute to the progression of primary gastric carcinomas possibly through immunosuppression.
背景
已有研究表明，免疫抑制细胞因子在多种癌症的肿瘤进展中发挥作用。为了明确免疫抑制细胞因子在胃癌中的体内表达情况，我们分析了人胃癌组织中转化生长因子-β（TGF-β）和白细胞介素-10（IL-10）的信使核糖核酸（mRNA）表达。
方法
采用逆转录聚合酶链反应（RT-PCR）检测29例原发性胃癌手术切除标本的肿瘤组织和非肿瘤组织中IL-10和TGF-β mRNA的表达，并将mRNA表达与肿瘤的各种病理参数进行关联分析。
结果
在29例肿瘤中，分别有79%和62%的肿瘤样本检测到TGF-β和IL-10的mRNA。在非肿瘤样本中，TGF-β和IL-10的检测率分别仅为31%和17%。两种mRNA在低分化腺癌以及高分期或有淋巴结转移的肿瘤组织中均频繁表达。
结论
免疫抑制细胞因子的局部表达可能通过免疫抑制作用促进原发性胃癌的进展。
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