Parfanovich M I, Zhdanov V M, Kolomiets A G, Nömm E M, Simovart Y A, Busol V A, Kolomiets N D, Lazarenko A A
Ann Rech Vet. 1978;9(4):781-96.
The results of five years studies on the possibility of experimental induction of bovine leukemia with purified BLV and different BLV-containing materials demonstrated that inoculation of calves with purified BLV (structures of 1.14-1.17 g/ml density in 20-60% sucrose gradient) can induce oncornavirus infection and bovine leukemia. From 41 experimentally infected calves 9 showed evidence of bovine leukemia. 5 of these 9 animals were from a group of 22 calves inoculated with purified BLV. Diagnosis of bovine leukemia in these 9 animals was established by hematologic (3 animals) and histologic method (6 animals). BLV was isolated from leukocytes of all of them. It was shown also that before the development of bovine leukemia the development of oncornavirus infection takes place. But only a small part of the animals with oncornavirus infection and presence of antibodies became ill with typical forms of hemoblastosis. The investigation of the role of milk and blood cells in milk in bovine leukemia transmission was also carried out. The presence was demonstrated of oncornavirus structures antigenically identical with BLV in milk and reproduction of these structures in blood cells being present in milk. These results, and also the induction of bovine leukemia, in cattle by rearing with raw milk from cattle with leukemia, allow us to suppose the possible routes of bovine leukemia transmission in nature.
对用纯化的牛白血病病毒（BLV）及不同含BLV材料进行实验诱导牛白血病可能性的五年研究结果表明，给犊牛接种纯化的BLV（在20 - 60%蔗糖梯度中密度为1.14 - 1.17 g/ml的结构）可诱发肿瘤病毒感染和牛白血病。在41头经实验感染的犊牛中，有9头显示出牛白血病的迹象。这9头动物中有5头来自接种纯化BLV的22头犊牛组。这9头动物的牛白血病诊断是通过血液学方法（3头动物）和组织学方法（6头动物）确定的。从所有这些动物的白细胞中都分离出了BLV。还表明在牛白血病发生之前会出现肿瘤病毒感染。但只有一小部分感染肿瘤病毒且存在抗体的动物患上典型的成血病形式。还对牛奶和牛奶中的血细胞在牛白血病传播中的作用进行了研究。已证明牛奶中存在与BLV抗原相同的肿瘤病毒结构，并且这些结构在牛奶中的血细胞中得以复制。这些结果以及通过用患白血病牛的生奶饲养牛而在牛中诱发牛白血病的情况，使我们能够推测出牛白血病在自然界中的可能传播途径。
