Rognlie M C, Dimke K L, Potts R S, Knapp S E
Veterinary Molecular Biology Laboratory, Montana State University-Bozeman 59717-0360, USA.
Vet Parasitol. 1996 Oct 25;65(3-4):297-305. doi: 10.1016/s0304-4017(96)00957-0.
A cashmere goat ranch in the Beaverhead Valley, southwest Montana, USA reported acute fasciolosis in March 1992. The ranch was used as a study site to gather seasonal transmission data for the liver fluke, Fasciola hepatica. Testing of snails for infection with a nucleic acid-based assay, and use of tracer sheep at the site has shown the seasonal transmission of the parasite is only in late autumn. The snail responsible for transmission was identified as Lymnaea modicella although another known intermediate host species, Lymnaea bulimoides, was found in one collection at the study site. Tracer sheep used over a 12-month period became infected with F. hepatica while on pasture during a period between September 10 and November 12, 1993. During the 28 months of study, 3072 individual lymnaeid snails were tested for infection. One sample of L. modicella containing 25 snails, collected in August 1994 contained liver fluke ribosomal RNA.
美国蒙大拿州西南部比弗黑德山谷的一个绒山羊牧场于1992年3月报告了急性片形吸虫病。该牧场被用作研究地点，以收集肝片吸虫（Fasciola hepatica）的季节性传播数据。用基于核酸的检测方法对蜗牛进行感染检测，并在该地点使用示踪羊，结果表明该寄生虫的季节性传播仅在深秋。虽然在研究地点的一次采集样本中发现了另一种已知的中间宿主物种——贪食椎实螺（Lymnaea bulimoides），但确定负责传播的蜗牛为小椎实螺（Lymnaea modicella）。在1993年9月10日至11月12日期间，在牧场放牧的示踪羊在12个月内感染了肝片吸虫。在28个月的研究期间，对3072只椎实螺个体进行了感染检测。1994年8月采集的一个含有25只小椎实螺的样本中含有肝片吸虫核糖体RNA。
