Il'ina A V, Bannikova G E, Varlamov V P, Agabekian R S
Prikl Biokhim Mikrobiol. 1996 Jul-Aug;32(4):389-92.
Acetylcholinesterase (AChE) was purified on columns with iminodiacetate Agarose charged with Cu2+, Zn2+, and Ni2+. The best results (a 14-fold purification and more than 30% activity yield) were obtained with Zn2+ used as a complex-forming metal. The preparation had a specific activity of approximately 7 U. Its purity was tested by polyacrylamide gel electrophoresis under denaturing conditions.
乙酰胆碱酯酶（AChE）在装填有Cu2+、Zn2+和Ni2+的亚氨基二乙酸琼脂糖柱上进行纯化。以Zn2+作为络合金属时获得了最佳结果（纯化了14倍，活性回收率超过30%）。该制剂的比活性约为7 U。在变性条件下通过聚丙烯酰胺凝胶电泳对其纯度进行了检测。
