[一种利用锎-252使分裂片段离子化进行质谱分析中蛋白质和肽探针制备的改进方法（飞行时间-质子动力质谱法）]
[An improved method of preparing protein and peptide probes in mass spectrometry with ionization of division fragments by californium-252 (TOF-PDMS)].
作者信息
Chivanov V D, Zubarev R A, Aksenov S A, Bordunova O G, Eremenko V I, Kabanets V M, Tatarinova V I, Mishnev A K, Kuraev V V, Knysh A N, Eremenko I A
出版信息
Bioorg Khim. 1996 Aug;22(8):585-8.
PMID:8985001
Abstract
The addition of organic acids (picric, oxalic, citric, or tartaric) to peptide and protein samples was found to significantly increase the yield of their quasi-molecular ions (QMI) in time-of-flight 252Cf plasma desorption mass spectrometry. The yield of the ions depended on the pKa of the acid added.
摘要
研究发现，在肽和蛋白质样品中添加有机酸（苦味酸、草酸、柠檬酸或酒石酸），可显著提高其在飞行时间252Cf等离子体解吸质谱中的准分子离子（QMI）产率。离子产率取决于所添加酸的pKa值。
相似文献
1
2
Californium-252 plasma desorption time of flight mass spectroscopy of proteins.
Biomed Mass Spectrom. 1984 May;11(5):242-57. doi: 10.1002/bms.1200110509.
3
4
Identification of protein-binding peptides by direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis of peptide beads selected from the screening of one bead-one peptide combinatorial libraries.
Anal Biochem. 2007 Nov 15;370(2):215-22. doi: 10.1016/j.ab.2007.07.032. Epub 2007 Aug 9.
5
Modified silver nanoparticle as a hydrophobic affinity probe for analysis of peptides and proteins in biological samples by using liquid-liquid microextraction coupled to AP-MALDI-ion trap and MALDI-TOF mass spectrometry.
Anal Chem. 2008 Apr 1;80(7):2583-9. doi: 10.1021/ac702309w. Epub 2008 Mar 7.
6
Californium-252 plasma desorption mass spectroscopy.
Science. 1976 Mar 5;191(4230):920-5. doi: 10.1126/science.1251202.
7
Mass spectrometric characterization of lipid-modified peptides for the analysis of acylated proteins.
J Mass Spectrom. 2006 Feb;41(2):229-41. doi: 10.1002/jms.981.
8
Quantitation of peptides and proteins by matrix-assisted laser desorption/ionization mass spectrometry using (18)O-labeled internal standards.
Rapid Commun Mass Spectrom. 2000;14(14):1226-32. doi: 10.1002/1097-0231(20000730)14:14<1226::AID-RCM14>3.0.CO;2-V.
9
Characterization of a synthetic 37-residue fragment of a monoclonal antibody against herpes virus by capillary electrophoresis/electrospray (ionspray) mass spectrometry and 252Cf plasma desorption mass spectrometry.
Biol Mass Spectrom. 1994 Jun;23(6):346-52. doi: 10.1002/bms.1200230608.
10
Comparison of laser-induced dissociation and high-energy collision-induced dissociation using matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) for peptide and protein identification.
Rapid Commun Mass Spectrom. 2004;18(18):2093-105. doi: 10.1002/rcm.1589.
Zotero 插件