Bronnenmeier K, Adelsberger H, Lottspeich F, Staudenbauer W L
Lehrstuhl für Mikrobiologie, Technische Universität München, Germany.
Bioseparation. 1996 Feb;6(1):41-5.
Cellulose-affinity chromatography proved to be a fast and efficient purification procedure for exoenzymes of the thermophilic anaerobe Clostridium stercorarium, suitable for the preparation of large amounts of enzymes for technical applications. The cellulose-binding enzymes could be identified as the C. stercorarium; cellulolytic enzymes Avicelase I and Avicelase II characterized previously as endo-1,4-beta-glucanase and exo-1,4-beta-glucanase. A third protein was identified as xylanase A, the major endo-1,4-beta-xylanase of this organism.
纤维素亲和层析被证明是一种用于嗜热厌氧菌粪堆梭菌外切酶的快速高效纯化方法，适用于制备大量用于技术应用的酶。纤维素结合酶可鉴定为粪堆梭菌的纤维素分解酶纤维二糖水解酶I和纤维二糖水解酶II，先前被表征为内切-1,4-β-葡聚糖酶和外切-1,4-β-葡聚糖酶。第三种蛋白质被鉴定为木聚糖酶A，是该生物体的主要内切-1,4-β-木聚糖酶。
