从缺乏伴大豆球蛋白α-和α'-亚基的突变体豆浆中基本完全去除34kDa致敏大豆蛋白Gly m Bd 30K。

Substantially complete removal of the 34kDa allergenic soybean protein, Gly m Bd 30 K, from soy milk of a mutant lacking the alpha- and alpha'-subunits of conglycinin.

作者信息

Samoto M, Takahashi K, Fukuda Y, Nakamura S, Kawamura Y

机构信息

Central Research Institute, Fuji Oil Co., Ltd, Ibaraki, Japan.

出版信息

Biosci Biotechnol Biochem. 1996 Nov;60(11):1911-3. doi: 10.1271/bbb.60.1911.

DOI:10.1271/bbb.60.1911

PMID:8987873

Abstract

Substantially complete removal (99.78%) of the allergenic soybean protein, Gly m Bd 30 K, was attained by applying simple centrifugation at 10,000 x g and at pH 4.5 in the presence of Na2SO4 to defatted soy milk from a genetically mutated soybean cultivar (Tohoku 124) which lacked the alpha- and alpha'-subunits of conglycinin. The removal rate was ten times higher than that (97.44%) from a normal soybean cultivar of I.O.M. grade.

摘要

通过在10,000×g离心力、pH值为4.5且存在Na2SO4的条件下，对来自缺乏伴大豆球蛋白α-和α'-亚基的基因突变大豆品种（东北124）的脱脂豆浆进行简单离心，可基本完全去除（99.78%）致敏性大豆蛋白Gly m Bd 30K。去除率比来自I.O.M.等级普通大豆品种的去除率（97.44%）高十倍。