罗氏Amplicor结核分枝杆菌（PCR）检测在呼吸道分泌物以外标本中的应用。

Application of the Roche Amplicor Mycobacterium tuberculosis (PCR) test to specimens other than respiratory secretions.

作者信息

D'Amato R F, Hochstein L H, Colaninno P M, Scardamaglia M, Kim K, Mastellone A J, Patel R C, Alkhuja S, Tevere V J, Miller A

机构信息

Department of Pathology, Catholic Medical Center of Brooklyn, New York, USA.

出版信息

Diagn Microbiol Infect Dis. 1996 Jan;24(1):15-7. doi: 10.1016/0732-8893(95)00256-1.

DOI:10.1016/0732-8893(95)00256-1

PMID:8988758

Abstract

The ability of the Roche AMPLICOR Mycobacterium tuberculosis (MTB) test to detect M. tuberculosis in specimens other than respiratory secretions was evaluated. A total of 249 specimens from 219 patients were tested. Of these, 12 specimens grew isolates of the M. tuberculosis complex and four grew isolates of the M. avium complex. The AMPLICOR MTB test was positive for 10 of the 12 specimens which grew M. tuberculosis and for three specimens which were culture negative. Two of the latter specimens were from patients with a clinical diagnosis of tuberculosis and with multiple sputum specimens which grew M. tuberculosis. Four specimens grew M. avium complex isolates, and all yielded negative AMPLICOR MTB test results. The sensitivity, specificity, and positive and negative predictive values for the AMPLICOR MTB test were 85.7%, 99.5%, 92.3%, and 99.1%, respectively. Our data indicate that the AMPLICOR MTB test will permit the rapid detection of M. tuberculosis in specimens other than respiratory secretions.

摘要

评估了罗氏AMPLICOR结核分枝杆菌（MTB）检测在呼吸道分泌物以外的标本中检测结核分枝杆菌的能力。对来自219名患者的总共249份标本进行了检测。其中，12份标本培养出结核分枝杆菌复合群菌株，4份标本培养出鸟分枝杆菌复合群菌株。在培养出结核分枝杆菌的12份标本中有10份以及3份培养阴性的标本，AMPLICOR MTB检测呈阳性。后两份标本中的两份来自临床诊断为结核病且多个痰标本培养出结核分枝杆菌的患者。4份标本培养出鸟分枝杆菌复合群菌株，所有这些标本的AMPLICOR MTB检测结果均为阴性。AMPLICOR MTB检测的敏感性、特异性、阳性预测值和阴性预测值分别为85.7%、99.5%、92.3%和99.1%。我们的数据表明，AMPLICOR MTB检测将能够快速检测呼吸道分泌物以外的标本中的结核分枝杆菌。