Dunphy C H
Department of Pathology, St. Louis University Health Sciences Center, MO 63104, USA.
Diagn Cytopathol. 1996 Dec;15(5):427-30. doi: 10.1002/(SICI)1097-0339(199612)15:5<427::AID-DC14>3.0.CO;2-P.
Combining cytomorphology and immunophenotypic data in evaluation of effusions for lymphomatous involvement is reliable, non-invasive, and expeditious. In this study, 30 effusion specimens from 30 patients with clinically suspected or previously diagnosed non-Hodgkin's lymphoma (NHL) were evaluated cytomorphologically and by flow cytometric immunophenotyping with a large panel of monoclonal antibodies. Of 11 patients with a previous diagnosis of NHL, 50% had cytomorphologic and immunophenotypic evidence of lymphomatous involvement of effusion specimens; therefore, flow cytometric immunophenotyping is recommended in these cases since there may be substantial therapeutic impact. In addition, 69% of the effusions positive for lymphomatous involvement represented newly diagnosed NHL; in 36% of these cases, tissue biopsy was not necessary since they were either high-grade (lymphoblastic or small, non-cleaved cell type) or of the small lymphocytic lymphoma cell type.
在评估积液是否存在淋巴瘤累及方面，结合细胞形态学和免疫表型数据是可靠、无创且快速的。在本研究中，对30例临床疑似或先前诊断为非霍奇金淋巴瘤（NHL）患者的30份积液标本进行了细胞形态学评估，并采用大量单克隆抗体通过流式细胞术免疫表型分析进行检测。在11例先前诊断为NHL的患者中，50%的积液标本有细胞形态学和免疫表型证据表明存在淋巴瘤累及；因此，在这些病例中建议进行流式细胞术免疫表型分析，因为这可能对治疗有重大影响。此外，69%的积液淋巴瘤累及阳性代表新诊断的NHL；在其中36%的病例中，由于它们要么是高级别（淋巴母细胞性或小无裂细胞型），要么是小淋巴细胞淋巴瘤细胞型，所以无需进行组织活检。
