Vinter-Jensen L, Juhl C O, Frystyk J, Dajani E Z, Oksbjerg N, Flyvbjerg A
Department of Clinical Biochemistry, KH Aarhus University Hospital, Denmark.
J Endocrinol. 1996 Dec;151(3):401-7. doi: 10.1677/joe.0.1510401.
It has recently been demonstrated that epidermal growth factor (EGF) administration to neonatal rodents causes growth retardation with concomitant reductions in circulation levels of IGF-I. We describe the effects of systemic EGF administration for 4 weeks on circulating levels of IGF-I and IGF-binding proteins (IGFBPs) and on thyroid hormones (tri-iodothyronine, T3; thyroxine, T4) in sexually mature pigs. Goettingen minipigs of either sex were treated with placebo (n = 5) or EGF (30 micrograms/kg per day, n = 6) s.c. for 4 weeks (in relation to an oesophageal sclerotherapy regimen). Blood samples were taken under anaesthesia before and after 1, 2, 3 and 4 weeks of treatment. Circulating levels of IGF-I, insulin, glucose, T3 and T4 were analysed every week and IGFBPs every second week. IGF-I was not reduced significantly after 1 week but significantly reduced after 2 and 3 weeks of EGF treatment. A similar decline was observed for the major IGFBP, IGFBP-3, which was reduced after 2 and 4 weeks. IGFBP-1, IGFBP-2 and IGFBP-4 increased throughout the treatment period (all significantly at week 4). EGF treatment induced increased circulating T3 after 2, 3 and 4 weeks of EGF treatment. In conclusion, we report that EGF treatment for 4 weeks in Goettingen minipigs reduces circulating IGF-I and IGFBP-3, increases circulating IGFBP-1, IGFBP-2 and IGFBP-4, and induces a slight hyperthyroidism as judged from increased circulating levels of T3.
最近有研究表明，对新生啮齿动物给予表皮生长因子（EGF）会导致生长迟缓，同时伴有胰岛素样生长因子-I（IGF-I）循环水平降低。我们描述了对性成熟猪进行为期4周的全身性EGF给药对IGF-I和IGF结合蛋白（IGFBPs）循环水平以及甲状腺激素（三碘甲状腺原氨酸，T3；甲状腺素，T4）的影响。选用不同性别的哥廷根小型猪，分别用安慰剂（n = 5）或EGF（30微克/千克/天，n = 6）皮下注射4周（参照食管硬化疗法方案）。在治疗1、2、3和4周前后的麻醉状态下采集血样。每周分析IGF-I、胰岛素、葡萄糖、T3和T4的循环水平，每两周分析IGFBPs。EGF治疗1周后IGF-I未显著降低，但在治疗2周和3周后显著降低。主要的IGFBP即IGFBP-3也出现类似下降，在治疗2周和4周后降低。IGFBP-1、IGFBP-2和IGFBP-4在整个治疗期间均升高（在第4周时均显著升高）。EGF治疗2、3和4周后导致循环T3升高。总之，我们报告在哥廷根小型猪中进行4周的EGF治疗会降低循环IGF-I和IGFBP-3，升高循环IGFBP-1、IGFBP-2和IGFBP-4，并从循环T3升高判断出诱导了轻度甲状腺功能亢进。
