Li Z, Upadhyaya N M, Kositratana W, Gibbs A J, Waterhouse P M
CSIRO Division of Plant Industry, Canberra, Australia.
J Gen Virol. 1996 Dec;77 ( Pt 12):3155-60. doi: 10.1099/0022-1317-77-12-3155.
The complete nucleotide sequence of the genome segment 5 (S5) of a Thai isolate of rice ragged stunt virus (RRSV) was determined. The 2682 nucleotide sequence contains a single long open reading frame capable of encoding a polypeptide with a molecular mass of approximately 91 kDa. Polypeptides encoded by various truncated cDNAs of S5 were expressed using the pGEX fusion protein vector and the highest level of fusion protein was obtained from a construct encoding a hydrophilic region of S5 protein. Antibodies raised against this fusion protein recognized a minor polypeptide, with a molecular mass of approximately 91 kDa, that was present in purified preparations of RRSV particles, infected insect vectors and infected rice plants. This indicates that RRSV S5 encodes a minor structural protein. Comparing the RRSV S5 sequence with sequences of other reoviruses did not reveal any significant sequence similarities.
测定了泰国水稻锯齿叶矮缩病毒（RRSV）分离株基因组片段5（S5）的完整核苷酸序列。该2682个核苷酸序列包含一个单一的长开放阅读框，能够编码一种分子量约为91 kDa的多肽。使用pGEX融合蛋白载体表达了由S5的各种截短cDNA编码的多肽，并且从编码S5蛋白亲水区域的构建体中获得了最高水平的融合蛋白。针对该融合蛋白产生的抗体识别出一种分子量约为91 kDa的次要多肽，该多肽存在于RRSV颗粒、受感染昆虫载体和受感染水稻植株的纯化制剂中。这表明RRSV S5编码一种次要结构蛋白。将RRSV S5序列与其他呼肠孤病毒的序列进行比较，未发现任何显著的序列相似性。
