Kline A E, Revilla V, Hernandez T D
Department of Psychology, University of Colorado at Boulder 80309-0345, USA.
Exp Neurol. 1997 Jan;143(1):124-31. doi: 10.1006/exnr.1996.6349.
Recently we reported that antecedent tone presentation significantly delays the rate of amygdala kindling when it precedes and overlaps with every kindling trial. The goal of the present study was to determine if there is a minimal number of pairings required for the tone to exert its effects or if continued exposure is required. To investigate this, male Long-Evans hooded rats were implanted with a bipolar electrode in the right amygdala and assigned to a Tone, No Tone, or Tone Discontinued group and kindled once daily. The Tone group was exposed to the auditory stimulus on every kindling trial, while the Tone Discontinued group received it for only the first 5 days and subsequently was kindled in the same manner as the No Tone group (i.e., not exposed to the tone while receiving the kindling stimulation). In agreement with our earlier report, antecedent tone presentation significantly delayed seizure progression for subjects kindled in the central nucleus. However, the antecedent tone also significantly accelerated epileptogenesis for rats kindled in the amygdalostriatal transition area and produced no significant difference for those kindled in the basolateral nucleus. Furthermore, presentation of the tone was not required with every kindling trial in order for these effects to be seen, suggesting that a critical period might exist early in the kindling process during which epileptogenesis is acutely vulnerable to intervention. Additional research is necessary to determine the nature of these interventions and what effects they may have on seizure genesis.
最近我们报道，当先行的音调呈现先于并与每次点燃试验重叠时，它会显著延迟杏仁核点燃的速率。本研究的目的是确定音调发挥其作用所需的配对次数是否存在最小值，或者是否需要持续暴露。为了对此进行研究，将雄性Long-Evans有帽大鼠右侧杏仁核植入双极电极，并分为音调组、无音调组或音调停止组，每天进行一次点燃。音调组在每次点燃试验时都接受听觉刺激，而音调停止组仅在前5天接受刺激，随后与无音调组以相同方式进行点燃（即在接受点燃刺激时不暴露于音调）。与我们早期的报告一致，先行的音调呈现显著延迟了在中央核点燃的受试者的癫痫发作进展。然而，先行的音调也显著加速了在杏仁核纹状体过渡区点燃的大鼠的癫痫发生，而对在基底外侧核点燃的大鼠则没有显著差异。此外，为了观察到这些效应，并不需要在每次点燃试验时都呈现音调，这表明在点燃过程早期可能存在一个关键期，在此期间癫痫发生极易受到干预。需要进行更多研究来确定这些干预的性质以及它们对癫痫发作产生可能有何种影响。
