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马苏大麻哈鱼的线粒体DNA序列分析——大麻哈鱼属的系统发育

Mitochondrial DNA sequence analysis of the masu salmon--phylogeny in the genus Oncorhynchus.

作者信息

Oohara I, Sawano K, Okazaki T

机构信息

National Research Institute of Aquaculture, Nansei, Mie, Japan.

出版信息

Mol Phylogenet Evol. 1997 Feb;7(1):71-8. doi: 10.1006/mpev.1996.0373.

DOI:10.1006/mpev.1996.0373
PMID:9007022
Abstract

We determined 2162-bp sequences from the mitochondrial genome of the masu salmon Oncorhynchus masou masou, chum salmon Oncorhynchus keta, and Atlantic salmon Salmo salar by using polymerase chain reactions. These DNA sequences span from the 3' region of the gene for ATPase subunit 6 to the 5' region of the gene for NADH dehydrogenase subunit 4L. With the aid of the sequence data from other Pacific salmonid species (Thomas and Beckenbach, 1989, J. Mol. Evol. 29: 233-245), the evolutionary distances among the masu salmon and other species in the genus Oncorhynchus were calculated. These evolutionary distances were then used to construct a neighbor-joining tree. Further, a maximum parsimony tree was constructed. The evolutionary trees that were obtained suggest that masu salmon first diverged from the common ancestor of the genus Oncorhynchus.

摘要

我们通过聚合酶链反应测定了马苏大麻哈鱼(Oncorhynchus masou masou)、狗鲑(Oncorhynchus keta)和大西洋鲑(Salmo salar)线粒体基因组的2162碱基对序列。这些DNA序列从ATP酶亚基6基因的3'区域延伸至NADH脱氢酶亚基4L基因的5'区域。借助其他太平洋鲑科物种的序列数据(Thomas和Beckenbach,1989年,《分子进化杂志》29:233 - 245),计算了马苏大麻哈鱼与大麻哈鱼属其他物种之间的进化距离。然后利用这些进化距离构建了邻接法树。此外,还构建了最大简约树。所得到的进化树表明,马苏大麻哈鱼首先从大麻哈鱼属的共同祖先中分化出来。

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