Oda K
Department of Preventive Medicine and Public Health, School of Medicine, Keio University, Tokyo, Japan.
Ind Health. 1997;35(1):61-8. doi: 10.2486/indhealth.35.61.
To clarify the instillation toxicity of low level of indium phosphide (InP), 0, 1.2, 6.0 and 62.0 micrograms/kg body weight of InP particles were instilled intratracheally in male Fischer 344 rats, and the effects of InP were examined on the following day (day 1) and on the 8th day (day 8) after instillation. Indium was measured but not detected in the serum, liver, kidney, spleen, thymus and brain. Dose-related mild elevation of superoxide dismutase (SOD) activity and lactate dehydrogenase (LDH) activity in bronchoalveolar lavage fluid (BALF) were found on day 1 without increases of inflammatory cells and total protein (TP) in BALF, which suggested the response of neutrophils and alveolar macrophages to instilled InP, and/or the manifestation of a very early stage of inflammation. Only in the 62.0 micrograms/kg-instilled group on day 8, were neutrophils, lymphocytes, TP, LDH, total phospholipid and total cholesterol in BALF increased, and desquamation of alveolar epithelial cells and amorphous exudate in alveolar lumen observed by histopathological examination. These results suggested that InP caused pulmonary inflammation and epithelial cell damage up to 8 days following instillation dose of 62.0 micrograms/kg, but that its effect was considered irrelevant at instillation doses of 6.0 micrograms/kg or below in rat.
为阐明低水平磷化铟（InP）的滴注毒性，将0、1.2、6.0和62.0微克/千克体重的InP颗粒经气管内滴注到雄性Fischer 344大鼠体内，并在滴注后的次日（第1天）和第8天（第8天）检查InP的作用。在血清、肝脏、肾脏、脾脏、胸腺和大脑中测量了铟，但未检测到。在第1天，支气管肺泡灌洗液（BALF）中的超氧化物歧化酶（SOD）活性和乳酸脱氢酶（LDH）活性出现与剂量相关的轻度升高，而BALF中的炎症细胞和总蛋白（TP）没有增加，这表明中性粒细胞和肺泡巨噬细胞对滴注的InP有反应，和/或处于炎症非常早期的表现。仅在第8天的62.0微克/千克滴注组中，BALF中的中性粒细胞、淋巴细胞、TP、LDH、总磷脂和总胆固醇增加，并且通过组织病理学检查观察到肺泡上皮细胞脱落和肺泡腔内无定形渗出物。这些结果表明，在滴注剂量为62.0微克/千克后长达8天，InP可引起肺部炎症和上皮细胞损伤，但在大鼠中，滴注剂量为6.0微克/千克或更低时，其影响被认为不相关。
