Seidel K E, Pfisterer C, Hörmansdorfer S, Bauer J
Lehrstuhl für Tierhygiene, Technische Universität München, Freising, Deutschland.
Zentralbl Veterinarmed B. 1996 Sep;43(7):421-8.
Investigations into optimal culture conditions for Bacillus cereus in order to detect bacterial toxins in a cell culture system showed that Dulbecco's modified Eagle's medium supplemented with 5% fetal calf serum is the medium best suited for this purpose. The highest toxicity levels were seen when bacteria were cultured at 21 degrees C. In 42 of 43 Bacillus cereus-strains isolated from milk and milk products, toxin formation was detected using the MTT-test. In 11 strains, toxin formation was even shown when bacteria were cultured at 4 degrees C. When culture supernatants were examined in a commercially available ELISA, all cytotoxic strains were shown to form diarrheal toxin.
为了在细胞培养系统中检测细菌毒素，对蜡样芽孢杆菌的最佳培养条件进行了研究，结果表明，添加5%胎牛血清的杜氏改良伊格尔培养基最适合此目的。当细菌在21摄氏度培养时，毒性水平最高。在从牛奶和奶制品中分离出的43株蜡样芽孢杆菌菌株中，有42株通过MTT试验检测到毒素形成。在11株菌株中，即使细菌在4摄氏度培养时也显示出毒素形成。当使用市售酶联免疫吸附测定法检测培养上清液时，所有细胞毒性菌株均显示形成腹泻毒素。
